Anti-MAP2抗体(ab75713)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MAP2抗体
参阅全部 MAP2 一抗 -
描述
鸡多克隆抗体to MAP2 -
宿主
Chicken -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Two synthetic peptidescorresponding to different regions of the MAP2 gene product, but shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences conjugated to KLH.
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常规说明
Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 0.0268% PBS
PBS isotonic (0.9%, w/v) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
IgY fractions purified from yolks then affinity purified using a peptide column. Equal volumes of both of these affinity purified anti peptide antibodies were mixed, and the preparation was filter sterilized. -
Primary antibody说明
Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/2000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/2000. |
靶标
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功能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
序列相似性
Contains 3 Tau/MAP repeats. -
翻译后修饰
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
细胞定位
Cytoplasm, cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 17756 Mouse
- SwissProt: P20357 Mouse
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
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别名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab75713)
Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP.
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab75713)Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP, Blue - DAPI nuclear stain.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab75713 被引用在 9 文献中.
- Boshans LL et al. Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2. Sci Rep 11:3552 (2021). PubMed: 33574458
- Liu L et al. Protective effects of the knockdown of lncRNA AK139328 against oxygen glucose deprivation/reoxygenation-induced injury in PC12 cells. Mol Med Rep 24:N/A (2021). PubMed: 34212979
- Wu M et al. Wnt signaling contributes to withdrawal symptoms from opioid receptor activation induced by morphine exposure or chronic inflammation. Pain 161:532-544 (2020). PubMed: 31738230
- Zhang X et al. Ischemia-induced upregulation of autophagy preludes dysfunctional lysosomal storage and associated synaptic impairments in neurons. Autophagy N/A:1-24 (2020). PubMed: 33111641
- Shu XS et al. Loss of ß-catenin via activated GSK3ß causes diabetic retinal neurodegeneration by instigating a vicious cycle of oxidative stress-driven mitochondrial impairment. Aging (Albany NY) 12:13437-13462 (2020). PubMed: 32575075
- Huang X et al. Non-invasive Low-Intensity Pulsed Ultrasound Modulates Primary Cilia of Rat Hippocampal Neurons. Ultrasound Med Biol 45:1274-1283 (2019). PubMed: 30795858
- Brouwer M et al. SALM1 controls synapse development by promoting F-actin/PIP2-dependent Neurexin clustering. EMBO J 38:e101289 (2019). PubMed: 31368584
- Zhu H et al. GSK3ß-mediated tau hyperphosphorylation triggers diabetic retinal neurodegeneration by disrupting synaptic and mitochondrial functions. Mol Neurodegener 13:62 (2018). PubMed: 30466464
- Ruiz-Palmero I et al. Oestradiol synthesized by female neurons generates sex differences in neuritogenesis. Sci Rep 6:31891 (2016). PubMed: 27553191