重组Anti-MAP1LC3A抗体[EP1528Y] (ab52628)
![Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.cn/ps/products/52/ab52628/Images/ab52628-64999-anti-map1lc3a-antibody-ep1528y-western-blot.jpg "Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1528Y] to MAP1LC3A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MAP1LC3A抗体[EP1528Y]
参阅全部 MAP1LC3A 一抗 -
描述
兔单克隆抗体[EP1528Y] to MAP1LC3A -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human MAP1LC3A aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9H492 -
阳性对照
- WB: Fetal brain cell lysate. IHC-P: Human hippocampus. ICC/IF: SH-SY5Y cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1528Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100.
|
|
| WB | (8) |
1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/100. |
|
WB
1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
组织特异性
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. -
序列相似性
Belongs to the MAP1 LC3 family. -
翻译后修饰
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
细胞定位
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
别名
- ATG8E antibody
- Autophagy related protein LC3 A antibody
- Autophagy related ubiquitin like modifier LC3 A antibody
see all
图片
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Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.cn/ps/products/52/ab52628/Images/ab52628-4-anti-map1lc3a-antibody-ep1528y-immunocytochemistry-shsy5y-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling MAP1LC3A with purified ab52628 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.cn/ps/products/52/ab52628/Images/ab52628-64997-anti-map1lc3a-antibody-ep1528y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.cn/ps/products/52/ab52628/Images/ab52628-5-benefits-of-recombinant-antibodies.png)
Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab52628 被引用在 14 文献中.
- Wei M et al. Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway. Drug Des Devel Ther 14:395-405 (2020). PubMed: 32099325
- Liang AL et al. Chloroquine increases the anti-cancer activity of epirubicin in A549 lung cancer cells. Oncol Lett 20:53-60 (2020). PubMed: 32565933
- Brun P et al. The regulatory activity of autophagy in conjunctival fibroblasts and its possible role in vernal keratoconjunctivitis. J Allergy Clin Immunol 146:1210-1213.e9 (2020). PubMed: 32272130
- Scaini G et al. TSPO upregulation in bipolar disorder and concomitant downregulation of mitophagic proteins and NLRP3 inflammasome activation. Neuropsychopharmacology 44:1291-1299 (2019). PubMed: 30575805
- Silva JAF et al. Macrophage roles in the clearance of apoptotic cells and control of inflammation in the prostate gland after castration. Prostate 78:95-103 (2018). PubMed: 29134671