重组Anti-MAP1A抗体[EPR18994] - BSA and Azide free (ab223151)
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)](https://www.abcam.cn/ps/products/223/ab223151/Images/ab223151-3-ab184350264688IF1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18994] to MAP1A - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-MAP1A抗体[EPR18994] - BSA and Azide free
参阅全部 MAP1A 一抗 -
描述
兔单克隆抗体[EPR18994] to MAP1A - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse embryo, brain and spinal cord lysates; Rat brain lysate; Neuro-2a and C6 whole cell lysates. ICC/IF: Neuro-2a and C6 cells. Flow Cyt (intra): Neuro-2a cells.
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常规说明
ab223151 is the carrier-free version of ab184350.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18994 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223151于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 300, 26 kDa (predicted molecular weight: 305 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 300, 26 kDa (predicted molecular weight: 305 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements. -
组织特异性
Brain. -
序列相似性
Belongs to the MAP1 family. -
结构域
The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
LC2 is generated from MAP1A by proteolytic processing. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 17754 Mouse
- Entrez Gene: 25152 Rat
- SwissProt: Q9QYR6 Mouse
- SwissProt: P34926 Rat
- Unigene: 36501 Mouse
- Unigene: 11402 Rat
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别名
- MAP 1A antibody
- MAP 1L antibody
- MAP-1A antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184350 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184350).
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![Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)](https://www.abcam.cn/ps/products/223/ab223151/Images/ab223151-2-ab184350264687IF2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184350 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184350).
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![Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)](https://www.abcam.cn/ps/products/223/ab223151/Images/ab223151-1-ab184350264686fitc.jpg)
Flow Cytometry (Intracellular) - Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]- Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184350).
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![Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)](https://www.abcam.cn/ps/products/223/ab223151/Images/ab223151-4-benefits-of-recombinant-antibodies.png)
Anti-MAP1A antibody [EPR18994] - BSA and Azide free (ab223151)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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