Anti-Mannan Binding Lectin/MBL抗体[14D12] (ab106046)
![Immunohistochemistry (Frozen sections) - Anti-Mannan Binding Lectin/MBL antibody [14D12] (ab106046)](https://www.abcam.cn/ps/products/106/ab106046/Images/ab106046-118397-anti-mannan-binding-lectin-antibody-14d12-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Mannan Binding Lectin/MBL antibody [14D12] (ab106046)")
Key features and details
- Rat monoclonal [14D12] to Mannan Binding Lectin/MBL
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Mannan Binding Lectin/MBL抗体[14D12]
参阅全部 Mannan Binding Lectin/MBL 一抗 -
描述
大鼠单克隆抗体[14D12] to Mannan Binding Lectin/MBL -
宿主
Rat -
特异性
The immunogen for this antibody is from Mouse MBL-2 (MBL-C).
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经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Mouse -
免疫原
Full length native protein (purified) corresponding to Mouse Mannan Binding Lectin/MBL. The immunogen for this antibody is from Mouse MBL-2 (MBL-C).
Database link: P41317 -
阳性对照
- IHC-P: Mouse Serum and kidney tissue
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常规说明
It is recommended that solutions with a calcium concentration of 1 mM are used as ab106046 is a calcium-dependent antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
0.2 µm filtered antibody solution -
克隆
单克隆 -
克隆编号
14D12 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
1/10.
Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum |
| 说明 |
|---|
|
IHC-Fr
1/10. Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum |
靶标
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功能
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
组织特异性
Plasma protein produced mainly in the liver. -
疾病相关
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
序列相似性
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 17195 Mouse
- SwissProt: P41317 Mouse
- Unigene: 30045 Mouse
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别名
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-Mannan Binding Lectin/MBL antibody [14D12] (ab106046)
ab106046 detecting Mannan Binding Lectin/MBL deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. Mannan Binding Lectin/MBL was detected in and around invading macrophages invading the intima (insert). Mannan Binding Lectin/MBL bound at sites of necrosis (upper right corner). No Mannan Binding Lectin/MBL binding was shown in the media or on fibrous caps covering the thickened intima.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab106046 被引用在 6 文献中.
- Bowie E & Goetz SC TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife 9:N/A (2020). PubMed: 31934864
- Garland DL et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). PubMed: 23943789
- Matthijsen RA et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 119:2188-95 (2009). PubMed: 19380618
- Held K et al. Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol 45:3934-41 (2008). PubMed: 18672286
- Phaneuf LR et al. Binding of mouse mannan-binding lectins to different bacterial pathogens of mice. Vet Immunol Immunopathol 118:129-33 (2007). PubMed: 17493687
- Liu H et al. Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scand J Immunol 53:489-97 (2001). PubMed: 11309157