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重组RabMAb

重组Anti-MANBA抗体[EPR27977-48] - BSA and Azide free (ab317497)

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Western blot - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Western blot - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
  • Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR27977-48] to MANBA - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-MANBA抗体[EPR27977-48] - BSA and Azide free
    参阅全部 MANBA 一抗

  • 描述

    兔单克隆抗体[EPR27977-48] to MANBA - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Unsuitable for rat WB.

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: ICC/IF or IP

  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human

  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse pancreas, Mouse lung, Mouse liver, Mouse kidney, RAW264.7, NMuLi and Beta-TC-6 lysates. IHC-P: Mouse kidney, Mouse liver, Rat kidney and Rat liver tissues.

  • 常规说明

    ab317497 is the carrier-free version of ab317496.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317497于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF or IP.

靶标

  • 功能

    Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

  • 通路

    Glycan metabolism; N-glycan degradation.

  • 疾病相关

    Defects in MANBA are the cause of a mild disorder that affects peripheral and central nervous system myelin.
    Mannosidosis, beta A, lysosomal

  • 序列相似性

    Belongs to the glycosyl hydrolase 2 family.

  • 细胞定位

    Lysosome.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Beta-mannosidase antibody
      • GC04M103771 antibody
      • Lysosomal beta A mannosidase antibody
      • Manba antibody
      • MANBA_HUMAN antibody
      • Mannanase antibody
      • Mannase antibody
      see all

    图片

    • Western blot - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Western blot - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      All lanes : Anti-MANBA antibody [EPR27977-48] (ab317496) at 1/1000 dilution

      Lane 1 : Mouse pancreas tissue lysate
      Lane 2 : Mouse lung tissue lysate
      Lane 3 : Mouse liver tissue lysate
      Lane 4 : Mouse kidney tissue lysate
      Lane 5 : Mouse skeletal muscle tissue lysate
      Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 101 kDa why is the actual band size different from the predicted?


      Exposure time: 125 seconds


      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: skeletal muscle(PMID:9384606), NIH/3T3

      The identity of the bands lower than 100 kDa are unknown.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Western blot - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      All lanes : Anti-MANBA antibody [EPR27977-48] (ab317496) at 1/1000 dilution

      Lane 1 : NMuLi (mouse liver epithelial) whole cell lysate
      Lane 2 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 101 kDa why is the actual band size different from the predicted?


      Exposure time: 125 seconds


      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse kidney (PMID: 33441424).
      The section was incubated with ab317496 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument
      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on mouse liver.
      The section was incubated with ab317496 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument
      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on rat kidney.
      The section was incubated with ab317496 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument
      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on rat liver.
      The section was incubated with ab317496 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument
      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression tissue: No staining on mouse skeletal muscle.
      The section was incubated with ab317496 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument
      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

      This data was developed using ab317496, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression tissue: No staining on rat skeletal muscle.
      The section was incubated with ab317496 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument
      Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
      Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317497？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317497 尚未被引用在任何文献中。

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