MANBA重组抗体[EPR27977-48]_MANBA抗体(ab317496)| Abcam中文官网

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Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR27977-48] to MANBA
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat

Carrier Free

产品名称

Anti-MANBA抗体[EPR27977-48]
参阅全部 MANBA 一抗
描述

兔单克隆抗体[EPR27977-48] to MANBA
宿主

Rabbit
特异性

Unsuitable for rat WB.
经测试应用

适用于: IHC-P, WBmore details
不适用于: ICC/IF or IP
种属反应性

与反应: Mouse, Rat
不与反应: Human
免疫原

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Mouse pancreas, Mouse lung, Mouse liver, Mouse kidney, RAW264.7, NMuLi and Beta-TC-6 lysates. IHC-P: Mouse kidney, Mouse liver, Rat kidney and Rat liver tissues.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR27977-48
同种型

IgG
研究领域

Alternative Versions
- Anti-MANBA antibody [EPR27977-48] - BSA and Azide free (ab317497)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)

The Abpromise guarantee

Abpromise™承诺保证使用ab317496于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000.

说明
IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000.

应用说明

Is unsuitable for ICC/IF or IP.

功能

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.
通路

Glycan metabolism; N-glycan degradation.
疾病相关

Defects in MANBA are the cause of a mild disorder that affects peripheral and central nervous system myelin.
Mannosidosis, beta A, lysosomal
序列相似性

Belongs to the glycosyl hydrolase 2 family.
细胞定位

Lysosome.
Target information above from: UniProt accession O00462 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 110173 Mouse
- Entrez Gene: 310864 Rat
- SwissProt: Q8K2I4 Mouse
- SwissProt: Q4FZV0 Rat
- Unigene: 280536 Mouse
- Unigene: 20578 Rat
别名
- Beta-mannosidase antibody
- GC04M103771 antibody
- Lysosomal beta A mannosidase antibody
- Manba antibody
- MANBA_HUMAN antibody
- Mannanase antibody
- Mannase antibody
see all

Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496)

All lanes : Anti-MANBA antibody [EPR27977-48] (ab317496) at 1/1000 dilution

Lane 1 : Mouse pancreas tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse skeletal muscle tissue lysate
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 7 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Observed band size: 101 kDa why is the actual band size different from the predicted?

Exposure time: 125 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Low expression: skeletal muscle(PMID:9384606), NIH/3T3

The identity of the bands lower than 100 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-MANBA antibody [EPR27977-48] (ab317496)

All lanes : Anti-MANBA antibody [EPR27977-48] (ab317496) at 1/1000 dilution

Lane 1 : NMuLi (mouse liver epithelial) whole cell lysate
Lane 2 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Observed band size: 101 kDa why is the actual band size different from the predicted?

Exposure time: 125 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse kidney (PMID: 33441424).
The section was incubated with ab317496 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse liver.
The section was incubated with ab317496 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat kidney.
The section was incubated with ab317496 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat liver.
The section was incubated with ab317496 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on mouse skeletal muscle.
The section was incubated with ab317496 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MANBA antibody [EPR27977-48] (ab317496)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MANBA with ab317496 at 1/800 (0.643 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on rat skeletal muscle.
The section was incubated with ab317496 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Anti-MANBA antibody [EPR27977-48] (ab317496)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab317496 尚未被引用在任何文献中。

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Related Products

应用

The Abpromise guarantee

靶标

功能

通路

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (0)

客户评价及客户问答