Anti-MAN1A2抗体(ab272611)
Key features and details
- Rabbit polyclonal to MAN1A2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MAN1A2抗体
参阅全部 MAN1A2 一抗 -
描述
兔多克隆抗体to MAN1A2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MAN1A2 aa 100-200.
Database link: O60476 -
阳性对照
- ICC/IF: A431. WB: RT4. IHC-P: Human skeletal tissue, kidney tissue, prostate tissue, placenta tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272611于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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相关性
MAN1A2 plays a role in the maturation of Asn-linked oligosaccharides (N-glycans) in mammalian cells, by progressively excising alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). -
细胞定位
Golgi apparatus membrane; Single-pass type II membrane protein -
数据库链接
- Entrez Gene: 10905 Human
- Omim: 604345 Human
- SwissProt: O60476 Human
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别名
- Alpha 1,2 mannosidase IB antibody
- MAN1B antibody
- Mannosidase alpha class 1A member 2 antibody
see all
图片
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Western blot - Anti-MAN1A2 antibody (ab272611)Lane 2 : Anti-MAN1A2 antibody (ab272611) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Human cell line RT4 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)Paraffin-embedded formalin-fixed Human skeletal tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis. Low expression as expected.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)Paraffin-embedded formalin-fixed Human kidney tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)Paraffin-embedded formalin-fixed Human prostate tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-MAN1A2 antibody (ab272611)PFA fixed, Triton X-100 permeablized human cell line A-431 labeling MAN1A2 (green) using ab272611at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAN1A2 antibody (ab272611)Paraffin-embedded formalin-fixed Human placenta tissue stained for MAN1A2 using ab272611 at 1/500 in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272611 尚未被引用在任何文献中。