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Anti-Maltose Binding蛋白抗体[R29.6] (ab65)

Reviews (2)Q&A (4)References (8)
Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)

    Key features and details

    • Mouse monoclonal [R29.6] to Maltose Binding Protein
    • Suitable for: IP, WB, ChIP
    • Reacts with: Escherichia coli
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Maltose Binding蛋白抗体[R29.6]
      参阅全部 Maltose Binding Protein 一抗

    • 描述

      小鼠单克隆抗体[R29.6] to Maltose Binding蛋白

    • 宿主

      Mouse

    • 经测试应用

      适用于: IP, WB, ChIPmore details

    • 种属反应性

      与反应: Escherichia coli

    • 免疫原

      Fusion protein corresponding to Maltose Binding Protein. MOS maltose binding protein fusion protein

    • 阳性对照

      • MBP fusion protein generated with the pmal plasmid (New England Biolabs) in bacterial lysate.

    • 常规说明

      MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...

    • 纯度

      Protein A purified

    • 克隆

      单克隆

    • 克隆编号

      R29.6

    • 骨髓瘤

      Sp2/0

    • 同种型

      IgG1

    • 轻链类型

      unknown

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab65于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IP
    Use at an assay dependent concentration.
    WB (1)
    1/10000.
    ChIP (1)
    Use at an assay dependent concentration.
    说明
    IP
    Use at an assay dependent concentration.
    WB
    1/10000.
    ChIP
    Use at an assay dependent concentration.

    靶标

    • 相关性

      Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

    • 细胞定位

      Periplasm

    • 数据库链接

      • 别名

        • ECK4026 antibody
        • JW3994 antibody
        • Mal E antibody
        • MalE antibody
        • malJ antibody
        • Maltodextrin binding protein antibody
        • Maltose ABC transporter periplasmic protein antibody
        • Maltose binding periplasmic protein antibody
        • MMBP antibody
        • Periplasmic maltose binding protein antibody
        see all

      图片

      • Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)
        Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.
        Representative western blot detecting Maltose Binding Protein using ab65 at 1.8 µg/ml.

      文献 (8)

      发表研究结果有使用 ab65？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab65 被引用在 8 文献中.

      • Li Y  et al. ABNORMAL SHOOT 6 interacts with KATANIN 1 and SHADE AVOIDANCE 4 to promote cortical microtubule severing and ordering in Arabidopsis. J Integr Plant Biol 63:646-661 (2021). PubMed: 32761943
      • Morrison MS  et al. Disulfide-compatible phage-assisted continuous evolution in the periplasmic space. Nat Commun 12:5959 (2021). PubMed: 34645844
      • Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). PubMed: 21057026
      • Im YJ  et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). PubMed: 19686684
      • Verhoeven GS  et al. Differential bacterial surface display of peptides by the transmembrane domain of OmpA. PLoS One 4:e6739 (2009). WB . PubMed: 19707582
      • Liu X  et al. Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection. Genome Res 16:1517-28 (2006). PubMed: 17053089
      • Liu X  et al. DIP-chip: rapid and accurate determination of DNA-binding specificity. Genome Res 15:421-7 (2005). ChIP ; Saccharomyces cerevisiae . PubMed: 15710749
      • Leliveld SR  et al. Apoptin induces tumor-specific apoptosis as a globular multimer. J Biol Chem 278:9042-51 (2003). ICC/IF . PubMed: 12496278

      客户评价及客户问答

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      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Maltose Binding Protein antibody [R29.6]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Bacteria Recombinant protein (BL-21)
      Gel Running Conditions
      Reduced Non-Denaturing (Native) (10%)
      Loading amount
      10 µg
      Specification
      BL-21
      Blocking step
      BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Jun Soung Kwak

      Verified customer

      提交于 Jan 09 2020

      ChIP abreview for Anti-Maltose Binding Protein antibody [R29.6] - ChIP Grade

       Excellent
      Abreviews
      Application
      ChIP
      Sample
      Saccharomyces cerevisiae Cell (S. cerevisae)
      Specification
      S. cerevisae
      Type
      Cross-linking (X-ChIP)
      Duration of cross-linking step: 0 second(s)
      Specification of the cross-linking agent: formaldehyde
      Detection step
      Other
      Positive control
      negative control: MBP-expressing vector in yeast
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Mr. Xiao Liu

      Verified customer

      提交于 Aug 11 2005

      Question

      What is the epitope recognized by product ab65?

      Read More
      Answer

      Unfortunately, the epitope recognized by product ab65 has not yet been mapped.

      Read More

      Question

      Do you know the gene which encodes the MBP to which these antibodies were raised? I am planning on getting a model where this gene is mutated, and use it as a negative control in my specific experiment.

      Read More
      Answer

      This antibody was raised against a fusion protein and unfortunately we don't have the amino acid sequence.

      Read More

      Question

      Can the anti-MBP (ref # ab65) be used in an ELISA assay? Second, can your company directly attach an HRP or AP conjugate directly to the anti-MBP? If so, how much does this cost?

      Read More
      Answer

      I am afraid that this antibody has not been tested yet in ELISA. We also don't currently provide a conjugation service for our antibodies, however we do supply a range of conjugated secondary antibodies that may be suitable for your needs.

      Read More

      Question

      At what dilution should one use the Anti-MBP (Ref. number: ab65)?

      Read More
      Answer

      The optimal dilution should be determined for each application , but a good start point for Western blots would be 1µg/ml

      Read More

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