Anti-Maltose Binding蛋白抗体[R29.6] (ab65)
![Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)](https://www.abcam.cn/ps/products/0/ab65/Images/ab65-3277-anti-maltose-binding-protein-antibody-r296-western-blot.jpg "Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)")
Key features and details
- Mouse monoclonal [R29.6] to Maltose Binding Protein
- Suitable for: IP, WB, ChIP
- Reacts with: Escherichia coli
- Isotype: IgG1
概述
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产品名称
Anti-Maltose Binding蛋白抗体[R29.6]
参阅全部 Maltose Binding Protein 一抗 -
描述
小鼠单克隆抗体[R29.6] to Maltose Binding蛋白 -
宿主
Mouse -
经测试应用
适用于: IP, WB, ChIPmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Fusion protein corresponding to Maltose Binding Protein. MOS maltose binding protein fusion protein
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阳性对照
- MBP fusion protein generated with the pmal plasmid (New England Biolabs) in bacterial lysate.
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常规说明
MBP is a bacterial protein, which is also used as a fusion protein in expression constructs.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
R29.6 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
靶标
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相关性
Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein. -
细胞定位
Periplasm -
数据库链接
- Entrez Gene: 1039564 Escherichia coli
- Entrez Gene: 4494002 Escherichia coli
- Entrez Gene: 948538 Escherichia coli
- Entrez Gene: 960089 Escherichia coli
- SwissProt: P0AEX9 Escherichia coli
- SwissProt: P0AEY0 Escherichia coli
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别名
- ECK4026 antibody
- JW3994 antibody
- Mal E antibody
see all
图片
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Western blot - Anti-Maltose Binding Protein antibody [R29.6] (ab65)Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.Representative western blot detecting Maltose Binding Protein using ab65 at 1.8 µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab65 被引用在 6 文献中.
- Kamennaya NA & Post AF Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. Appl Environ Microbiol 77:291-301 (2011). PubMed: 21057026
- Im YJ et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). PubMed: 19686684
- Verhoeven GS et al. Differential bacterial surface display of peptides by the transmembrane domain of OmpA. PLoS One 4:e6739 (2009). WB . PubMed: 19707582
- Liu X et al. Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection. Genome Res 16:1517-28 (2006). PubMed: 17053089
- Liu X et al. DIP-chip: rapid and accurate determination of DNA-binding specificity. Genome Res 15:421-7 (2005). ChIP ; Saccharomyces cerevisiae . PubMed: 15710749
- Leliveld SR et al. Apoptin induces tumor-specific apoptosis as a globular multimer. J Biol Chem 278:9042-51 (2003). ICC/IF . PubMed: 12496278
