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AB180505

重组Anti-MAGOH抗体[EPR14037]

Anti-MAGOH+MAGOHB antibody [EPR14037]

5

(5 Reviews)

|

(1 Publication)

Rabbit Recombinant Monoclonal MAGOH antibody. Suitable for WB, IHC-P, Dot and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

查看别名

MAGOHA, MAGOH, Protein mago nashi homolog

3 Images
Western blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)
  • WB

Supplier Data

Western blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (ab180505) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HL60 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling Mago nashi homolog 2 with ab180505 at 1/250 dilution. Secondary ab : HRP Polymer for Rabbit IgG. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Dot Blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)
  • Dot

Lab

Dot Blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (AB180505)

Dot blot analysis of MAGOH+MAGOHB using ab180505 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody cross-react with human MAGOH.

All lanes:

Dot Blot - Anti-MAGOH+MAGOHB antibody [EPR14037] (ab180505)

Lane 1:

Human MAGOHB protein

Lane 2:

Human MAGOH protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

不同偶联物与剂型 (1)

  • Carrier free

    Anti-MAGOH+MAGOHB antibody [EPR14037] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14037

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, Dot, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

ab180505 detects both Mago nashi homolog and Mago nashi homolog 2

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 11991638). Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway (PubMed : 23917022). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S pre-initiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
See full target information MAGOH

其他靶点

MAGOHB
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 72:496-509.e9 PubMed30388411

2018

Exon Junction Complex Shapes the Transcriptome by Repressing Recursive Splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Lorea Blazquez,Warren Emmett,Rupert Faraway,Jose Mario Bello Pineda,Simon Bajew,Andre Gohr,Nejc Haberman,Christopher R Sibley,Robert K Bradley,Manuel Irimia,Jernej Ule
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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关联产品

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