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AB71786

Anti-MAGEA2抗体- Aminoterminal end

Anti-MAGEA2 antibody - Aminoterminal end

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(1 Publication)

Rabbit Polyclonal MAGEA2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAGEA2 conjugated to Keyhole Limpet Haemocyanin.

查看别名

MAGE2, MAGEA2A, MAGEA2B, MAGEA2, Melanoma-associated antigen 2, Cancer/testis antigen 1.2, MAGE-2 antigen, CT1.2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA2 antibody - Aminoterminal end (AB71786)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA2 antibody - Aminoterminal end (AB71786)

Formalin fixed, paraffin embedded human breast carcinoma tissue reacted with ab71786 at a 1/50 dilution. ab71786 was peroxidase conjugated to the secondary antibody, followed by AEC staining.

Western blot - Anti-MAGEA2 antibody - Aminoterminal end (AB71786)
  • WB

Unknown

Western blot - Anti-MAGEA2 antibody - Aminoterminal end (AB71786)

All lanes:

Western blot - Anti-MAGEA2 antibody - Aminoterminal end (ab71786) at 1/100 dilution

All lanes:

HL60 lysate at 12.5 µg

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human MAGEA2 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P43356

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
纯化说明
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
存储溶液
Preservative: 0.09% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

MAGEA2 also known as Melanoma-associated antigen A2 is a member of the MAGE gene family located on the X chromosome. This protein has a mass of approximately 35 kDa. MAGEA2 expresses in a wide range of cancers and is usually absent in normal tissues except in the testis and placenta. This makes it a significant target for cancer research particularly in developing cancer vaccines and immunotherapies.
Biological function summary

MAGEA2 plays a role in regulating the p53 tumor suppressor pathway. It interacts with other proteins to inhibit p53 activity impacting cell cycle control and apoptosis. MAGEA2 is not generally part of a larger macromolecular complex but it can interact with several proteins that modulate its effects on cellular processes.

Pathways

Focusing on cell cycle and apoptosis reveals that MAGEA2 participates in inhibiting the p53 pathway. This suppression helps cancer cells evade normal growth control mechanisms. In addition to p53 MAGEA2 associates with proteins such as HDAC3 involved in transcriptional regulation. These interactions make it an intriguing target for modulating key pathways involved in cell proliferation and survival.

MAGEA2 shows strong associations with various cancers including melanoma and lung cancer. Its ability to suppress p53 activity connects it to the pathogenesis of these diseases where normal p53 function is often compromised. MAGEA2's interaction with proteins like HDAC3 also implies that targeting this network can potentially reverse aberrant epigenetic modifications seen in these cancers offering a therapeutic avenue.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Reduces p53/TP53 transactivation function through recruitment of HDAC3 to p53/TP53 transcription sites. Also represses p73/TP73 activity. Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination by TRIM28 potentially in presence of Ubl-conjugating enzyme UBE2H. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3 : substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Negatively regulates acetylation and sumoylation of PML and represses PML-induced p53/TP53 acetylation and activation.
See full target information MAGEA2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 15:2113-35 PubMed25900405

2015

Comparative proteomic analyses of two reovirus T3D subtypes and comparison to T1L identifies multiple novel proteins in key cellular pathogenic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia R Berard,Alberto Severini,Kevin M Coombs
View all publications
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