Anti-Mael抗体(ab106713)
Key features and details
- Rabbit polyclonal to Mael
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Mael抗体 -
描述
兔多克隆抗体to Mael -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
A 16 amino acid peptide near the C terminus of Human Mael (NP_065199).
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阳性对照
- Human skeletal muscle tissue; HeLa cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106713于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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| ICC |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
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ICC
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 20 µg/ml. |
靶标
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功能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage. -
组织特异性
Testis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter. -
序列相似性
Belongs to the maelstrom family.
Contains 1 HMG box DNA-binding domain. -
细胞定位
Cytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 84944 Human
- Omim: 611368 Human
- SwissProt: Q96JY0 Human
- Unigene: 651245 Human
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别名
- cancer/testis antigen 128 antibody
- CT128 antibody
- FLJ14904 antibody
see all
图片
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Western blot - Anti-Mael antibody (ab106713)Anti-Mael antibody (ab106713) at 1 µg/ml + HeLa cell lysate at 15 µg
Predicted band size: 49 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Mael antibody (ab106713)Immunofluorescence of Mael in HeLa cells with ab106713 at 20 ug/mL.
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Immunocytochemistry - Anti-Mael antibody (ab106713)ab106713, at 5 µg/ml, staining in Hela cells with Maelstrom by Immunocytochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab106713 被引用在 4 文献中.
- Wang Q et al. Morphine suppresses the immune function of lung cancer by up-regulating MAEL expression. BMC Pharmacol Toxicol 23:92 (2022). PubMed: 36476246
- Rocha-da-Silva L et al. Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics. PLoS One 14:e0217941 (2019). PubMed: 31181099
- Bothun AM et al. Quantitative Proteomic Profiling of the Human Ovary from Early to Mid-Gestation Reveals Protein Expression Dynamics of Oogenesis and Folliculogenesis. Stem Cells Dev 27:723-735 (2018). PubMed: 29631484
- Cao J et al. High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-ß receptors and cyclin-dependent kinases in breast cancer. Mol Med Rep 13:2829-35 (2016). PubMed: 26847393