Anti-MADH7/SMAD7抗体(ab272928)
Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MADH7/SMAD7抗体
参阅全部 MADH7/SMAD7 一抗 -
描述
兔多克隆抗体to MADH7/SMAD7 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MADH7/SMAD7 (N terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: O15105 -
阳性对照
- IHC-Fr: Mouse cerebellum tissue. ICC/IF: Rat E18 primary cortical neuron cells. WB: DDDDK-tagged MADH7 transfected HEK-293T whole cell extract. U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272928于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/10000.
|
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| IHC-Fr |
1/100 - 1/1000.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/500 - 1/10000. |
|
IHC-Fr
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
靶标
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功能
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. -
组织特异性
Ubiquitous with higher expression in the lung and vascular endothelium. -
疾病相关
Colorectal cancer 3 -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1. -
细胞定位
Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). - Information by UniProt
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数据库链接
- Entrez Gene: 4092 Human
- Entrez Gene: 17131 Mouse
- Entrez Gene: 81516 Rat
- Omim: 602932 Human
- SwissProt: O15105 Human
- SwissProt: O35253 Mouse
- SwissProt: O88406 Rat
- Unigene: 465087 Human
see all -
别名
- CRCS3 antibody
- FLJ16482 antibody
- hSMAD 7 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-MADH7/SMAD7 antibody (ab272928)
Frozen sections of mouse cerebellum tissue stained for MADH7/SMAD7 using ab272928 at 1/250 dilution in immunohistochemical analysis (green). Red: NF-H. Blue: DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-MADH7/SMAD7 antibody (ab272928)4% PFA-fixed rat E18 primary cortical neuron cells stained for MADH7/SMAD7 using ab272928 at 1/500 dilution in ICC/IF (green). Red: Tau. Blue: DAPI.
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Western blot - Anti-MADH7/SMAD7 antibody (ab272928)All lanes : Anti-MADH7/SMAD7 antibody (ab272928) at 1/5000 dilution
Lane 1 : Mock transfected HEK-293T cell lysate
Lane 2 : MAD1-transfected HEK-293T cell lysate
Lane 3 : SMAD2 transfected HEK-293T cell lysate
Lane 4 : SMAD3 transfected HEK-293T cell lysate
Lane 5 : SMAD4 transfected HEK-293T cell lysate
Lane 6 : SMAD5 transfected HEK-293T cell lysate
Lane 7 : SMAD7 transfected HEK-293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-MADH7/SMAD7 antibody (ab272928)All lanes : Anti-MADH7/SMAD7 antibody (ab272928) at 1/2000 dilution
Lane 1 : U-87 MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272928 被引用在 1 文献中.
- Jin Y & Zhang A Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7-TGF-ß pathway in allergic rhinitis. Mol Med Rep 25:N/A (2022). PubMed: 35029288