重组Anti-MAdCAM1抗体[EPR6946] (ab134088)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6946] to MAdCAM1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MAdCAM1抗体[EPR6946]
参阅全部 MAdCAM1 一抗 -
描述
兔单克隆抗体[EPR6946] to MAdCAM1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human MAdCAM1 aa 1-100 (extracellular). The exact sequence is proprietary.
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阳性对照
- HeLa, HUVEC, and ECV-304 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.84 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6946 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134088于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa). |
靶标
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功能
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. -
组织特异性
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. -
序列相似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
翻译后修饰
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8174 Human
- Omim: 102670 Human
- SwissProt: Q13477 Human
- Unigene: 102598 Human
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别名
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
图片
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All lanes : Anti-MAdCAM1 antibody [EPR6946] (ab134088) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab134088 尚未被引用在任何文献中。