重组Anti-MAD1L1/MAD1抗体[EPR14676] (ab184175)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14676] to MAD1L1/MAD1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MAD1L1/MAD1抗体[EPR14676]
参阅全部 MAD1L1/MAD1 一抗 -
描述
兔单克隆抗体[EPR14676] to MAD1L1/MAD1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, MCF7, HeLa and A431 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR14676 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184175于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
靶标
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功能
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. -
组织特异性
Expressed weakly at G0/G1 and highly at late S and G2/M phase. -
疾病相关
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. -
序列相似性
Belongs to the MAD1 family. -
翻译后修饰
Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
细胞定位
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. - Information by UniProt
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数据库链接
- Entrez Gene: 8379 Human
- Entrez Gene: 17120 Mouse
- Entrez Gene: 680006 Rat
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- SwissProt: Q9WTX8 Mouse
- Unigene: 654838 Human
- Unigene: 27250 Mouse
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别名
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
图片
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All lanes : Anti-MAD1L1/MAD1 antibody [EPR14676] (ab184175) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa -
All lanes : Anti-MAD1L1/MAD1 antibody [EPR14676] (ab184175) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 83 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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