Anti-Macrophages + Monocytes抗体[ER-HR3] (ab59697)
Key features and details
- Rat monoclonal [ER-HR3] to Macrophages + Monocytes
- Suitable for: Flow Cyt, IHC-Fr, IHC-P
- Reacts with: Mouse
- Isotype: IgG2c
概述
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产品名称
Anti-Macrophages + Monocytes抗体[ER-HR3] -
描述
大鼠单克隆抗体[ER-HR3] to Macrophages + Monocytes -
宿主
Rat -
特异性
This antibody is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are negative, up to 70% of circulating monocytes stain positive with this antibody. About 30% of bone marrow cells express the antigen. Other positive macrophages can be found in the splenic red pulp, in the mesentric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen. -
经测试应用
适用于: Flow Cyt, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Macrophages + Monocytes.
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常规说明
This antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified antibody in 0.2µm filtered solution. -
Primary antibody说明
This antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites. -
克隆
单克隆 -
克隆编号
ER-HR3 -
同种型
IgG2c -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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相关性
Monocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations. Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab59697 被引用在 2 文献中.
- Vidyasagar A et al. Tubular expression of heat-shock protein 27 inhibits fibrogenesis in obstructive nephropathy. Kidney Int 83:84-92 (2013). PubMed: 22971995
- Van Beneden K et al. Valproic acid attenuates proteinuria and kidney injury. J Am Soc Nephrol 22:1863-75 (2011). IHC-P ; Mouse . PubMed: 21868496
