Anti-M6PR (cation independent)抗体(ab12894)
Key features and details
- Rabbit polyclonal to M6PR (cation independent)
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-M6PR (cation independent)抗体
参阅全部 M6PR (cation independent) 一抗 -
描述
兔多克隆抗体to M6PR (cation independent) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Monkey, African green monkey
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免疫原
Synthetic peptide corresponding to Human M6PR (cation independent) aa 700-800 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab13210) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12894于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 4 µg/ml. This antibody worked well in IF in HeLa cells but not in SK-N-SH cells. |
靶标
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功能
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. -
序列相似性
Belongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain. -
结构域
Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends. -
细胞定位
Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 3482 Human
- Entrez Gene: 16004 Mouse
- Omim: 147280 Human
- SwissProt: P11717 Human
- SwissProt: Q07113 Mouse
- Unigene: 487062 Human
- Unigene: 673278 Human
- Unigene: 26553 Mouse
see all -
别名
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image was submitted as part of a review by Kirk McManus, University of British Columbia
HeLa cells labelled with anti-mannose-6-phosphate receptor (ab12894). ab12894 was used at a working concentration of 4µg/ml. The arrows indicate the perinuclear location of the mannose-6-phosphate receptor in the late endosomes. In the final panel DAPI is pseudo-coloured red, while the mannose-6-phosphate receptor is in green.
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Immunocytochemistry/ Immunofluorescence - Anti-M6PR (cation independent) antibody (ab12894)This image is courtesy of an anonymous Abreviewab12894 at 1/100 staining human MCF-7 cells by ICC/IF. The cells were methanol fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit FITC was used as the secondary.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab12894 被引用在 5 文献中.
- Fong LK et al. Full-length amyloid precursor protein regulates lipoprotein metabolism and amyloid-ß clearance in human astrocytes. J Biol Chem 293:11341-11357 (2018). PubMed: 29858247
- Liu Y et al. Endocytic processing of adeno-associated virus type 8 vectors for transduction of target cells. Gene Ther : (2012). Human . PubMed: 22622241
- Shen Y et al. Mutations in PNKD causing paroxysmal dyskinesia alters protein cleavage and stability. Hum Mol Genet 20:2322-32 (2011). ICC/IF ; Human . PubMed: 21487022
- Nishitsuji K et al. The E693Delta mutation in amyloid precursor protein increases intracellular accumulation of amyloid beta oligomers and causes endoplasmic reticulum stress-induced apoptosis in cultured cells. Am J Pathol 174:957-69 (2009). ICC/IF ; African green monkey . PubMed: 19164507
- Clarke JH et al. Distribution and neuronal expression of phosphatidylinositol phosphate kinase IIgamma in the mouse brain. J Comp Neurol 517:296-312 (2009). PubMed: 19757494