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重组RabMAb

重组Anti-M-CSF抗体[EP1179Y] (ab52864)

Submit a review Q&A (1)References (23)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
  • Anti-M-CSF antibody [EP1179Y] (ab52864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1179Y] to M-CSF
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

概述

  • 产品名称

    Anti-M-CSF抗体[EP1179Y]
    参阅全部 M-CSF 一抗

  • 描述

    兔单克隆抗体[EP1179Y] to M-CSF

  • 宿主

    Rabbit

  • 特异性

    This antibody fails to detect endogenous natural samples in WB.

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IP, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human, Recombinant fragment
    预测可用于: Sheep, Cow, Chimpanzee

  • 免疫原

    Synthetic peptide within Human M-CSF aa 50-150. The exact sequence is proprietary.
    Database link: P09603

  • 阳性对照

    • WB: Recombinant MCSF IHC-P: Human tonsil tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab52864于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/80.
WB
1/2000. Predicted molecular weight: 60 kDa.

This antibody fails to detect endogenous natural samples in WB.
IP
1/40.

For unpurified use at 1/50 dilution. 
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100 dilution.
ICC/IF
1/100.

For unpurified use at 1/250 - 1/500 dilution.
说明
Flow Cyt (Intra)
1/80.
WB
1/2000. Predicted molecular weight: 60 kDa.

This antibody fails to detect endogenous natural samples in WB.
IP
1/40.

For unpurified use at 1/50 dilution. 
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100 dilution.
ICC/IF
1/100.

For unpurified use at 1/250 - 1/500 dilution.

靶标

  • 功能

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.

  • 翻译后修饰

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.

  • 细胞定位

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Colony stimulating factor 1 (macrophage) antibody
      • Colony stimulating factor 1 antibody
      • Colony stimulating factor macrophage specific antibody
      • CSF 1 antibody
      • CSF-1 antibody
      • CSF1 antibody
      • CSF1_HUMAN antibody
      • Csfm antibody
      • Lanimostim antibody
      • M CSF antibody
      • M-CSF antibody
      • Macrophage Colony Stimulating Factor 1 antibody
      • Macrophage colony stimulating factor antibody
      • MCSF antibody
      • MGC31930 antibody
      • Processed macrophage colony-stimulating factor 1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling M-CSF with Purified ab52864 at 1:500 dilution (1.52 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunocytochemistry/ Immunofluorescence - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Immunocytochemistry/ Immunofluorescence analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with Purified ab52864 at 1:100 (7.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/2000 dilution (Purified) + Human M-CSF (aa 33-190) recombinant protein at 0.015 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 60 kDa
      Observed band size: 19 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Flow Cytometry (Intracellular) - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Intracellular Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling M-CSF with purified ab52864 at 1/80 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr®488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-M-CSF antibody [EP1179Y] (ab52864)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for M-CSF with unpurified ab52864  (1/50 dilution) in immunohistochemical analysis.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Western blot - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Anti-M-CSF antibody [EP1179Y] (ab52864) at 1/50000 dilution (unpurified) + recombinant MCSF at 0.005 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 60 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)
      Immunoprecipitation - Anti-M-CSF antibody [EP1179Y] (ab52864)

      ab52864 (purified) at 1:40 dilution (2µg) immunoprecipitating M-CSF in THP-1 whole cell lysate.
      Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg
      Lane 2 (+): ab52864 & THP-1 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52864 in THP-1 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-M-CSF antibody [EP1179Y] (ab52864)
      Anti-M-CSF antibody [EP1179Y] (ab52864)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (23)

    发表研究结果有使用 ab52864？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab52864 被引用在 23 文献中.

    • Nakajima S  et al. Neoadjuvant Chemotherapy Induces IL34 Signaling and Promotes Chemoresistance via Tumor-Associated Macrophage Polarization in Esophageal Squamous Cell Carcinoma. Mol Cancer Res 19:1085-1095 (2021). PubMed: 33674443
    • Back J  et al. Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction. Bone Res 9:43 (2021). PubMed: 34588427
    • Steins A  et al. High-grade mesenchymal pancreatic ductal adenocarcinoma drives stromal deactivation through CSF-1. EMBO Rep 21:e48780 (2020). PubMed: 32173982
    • Cui B  et al. CSF1R methylation is a key regulatory mechanism of tumor-associated macrophages in hepatocellular carcinoma. Oncol Lett 20:1835-1845 (2020). PubMed: 32724427
    • Lin H  et al. Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition. Aging (Albany NY) 12:12684-12702 (2020). PubMed: 32629426
    View all Publications for this product

    客户评价及客户问答

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    Question

    I've been using your antibody against M-CSF (ab52864). It says it is a 60kDa protein but is observed at 20kDa. When tested in my samples, this protein is coming up at around 45kDa. Have you seen this before? It is a very clean blot with only one specific band present so I do believe the antibody is very specific but just wondering if you have any advice in this case?


     



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    Answer

    I can confirm to you that it is reported in literature that M-CSF can also be detected in a 45 kDa form (see links below)

    https://www.ncbi.nlm.nih.gov/pubmed/2179407

    https://www.researchgate.net/publication/14333823_Macrophage_colony_stimulating_factor_involvement_in_uremic_patients  (in the introduction section).

    The size of the band is dependent on whether you are detecting the circulating or membrane bound form.

    I hope you found this information helpful and I am happy to answer any further questions or concerns you have.  The antibody is covered by the Abpromise guarantee.

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