Anti-M-CSF抗体(ab9693)
Key features and details
- Rabbit polyclonal to M-CSF
- Suitable for: WB, IHC-P, ELISA, Neutralising, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-M-CSF抗体
参阅全部 M-CSF 一抗 -
描述
兔多克隆抗体to M-CSF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ELISA, Neutralising, Sandwich ELISAmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human M-CSF. Highly pure (>98%) recombinant hM-CSF (human M-CSF)
Database link: P09603 -
阳性对照
- WB: Recombinant human M-CSF protein (ab9694).
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常规说明
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab52864
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200µl of sterile water. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
No preservative, sterile filtered -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
unknown -
轻链类型
unknown -
研究领域
相关产品
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. |
|
| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required. |
|
| Sandwich ELISA |
Use a concentration of 0.1 µg/ml.
To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
IHC-P
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required. |
|
Sandwich ELISA
Use a concentration of 0.1 µg/ml. To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. |
靶标
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功能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
翻译后修饰
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
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别名
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
图片
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Western blot - Anti-M-CSF antibody (ab9693)
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Western blot - Anti-M-CSF antibody (ab9693)This image is courtesy of an Abreview submitted by Valentina MarcelliAll lanes : Anti-M-CSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)
Lane 1 : Human Kidney whole cell lysate
Lane 2 : Human Kidney whole cell lysate transfected with LFABP
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution
Performed under non-reducing conditions.
Observed band size: 70 kDa why is the actual band size different from the predicted?
Blocking Step: 5% BSA for 1 hour at 25°C
Deatection method: Odyssey for 50 seconds.
Right-hand Image: Weaker exposure to identify bands. -
Sandwich ELISA - Anti-M-CSF antibody (ab9693)
数据表及文件
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Datasheet download
文献 (20)
ab9693 被引用在 20 文献中.
- Kurkipuro J et al. Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds. PLoS One 17:e0264775 (2022). PubMed: 35226700
- Huang YH et al. CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis. Signal Transduct Target Ther 6:10 (2021). PubMed: 33431788
- Liu D et al. CSF1-associated decrease in endometrial macrophages may contribute to Asherman's syndrome. Am J Reprod Immunol 83:e13191 (2020). PubMed: 31536655
- Sidorkiewicz I et al. Plasma Levels and Tissue Expression of Selected Cytokines, Metalloproteinases and Tissue Inhibitors in Patients With Cervical Cancer. Anticancer Res 39:6403-6412 (2019). PubMed: 31704874
- Chen X et al. CircRNA_28313/miR-195a/CSF1 axis modulates osteoclast differentiation to affect OVX-induced bone absorption in mice. RNA Biol 16:1249-1262 (2019). PubMed: 31204558
