Anti-LYVE1抗体- BSA and Azide free (ab14917)
Key features and details
- Rabbit polyclonal to LYVE1 - BSA and Azide free
- Suitable for: ICC, IHC-P
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LYVE1抗体- BSA and Azide free
参阅全部 LYVE1 一抗 -
描述
兔多克隆抗体to LYVE1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment (His-tag) within Mouse LYVE1 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8BHC0 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Protein-A Chromatography (+his tag depleted). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14917于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| IHC-P | (27) |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
组织特异性
Mainly expressed in endothelial cells lining lymphatic vessels. -
序列相似性
Contains 1 Link domain. -
翻译后修饰
O-glycosylated. -
细胞定位
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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数据库链接
- Entrez Gene: 114332 Mouse
- SwissProt: Q8BHC0 Mouse
- Unigene: 396078 Mouse
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别名
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue staining LYVE-1 with ab14917. Positive staining is shown in the lymphatic endothelial cells (red).
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Immunocytochemistry - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Immunocytochemistry/immunofluorecent analysis of mouse colon tissue labelling LYVE-1 with ab14917 (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Divan et al PLoS One. 2018 May 3;13(5):e0196893. doi: 10.1371/journal.pone.0196893. eCollection 2018. Fig S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/At the time point of 75dpi spirochetes (Bb) were not observed in association with the lymphatic-like vessels (LYVE-1) that run parallel to the sagittal sinus (SS, arrow) of the dura mater.
Dura samples were collected from transcardially perfused mice by craniotomy and post-fixed in 4% paraformaldehyde for 24h at 4°C. Samples were permeabilized in 0.1% Triton X-100, washed 3 times, and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block. For B. burgdorferi staining, each sample was incubated in 1:100 dilution of rat anti-mouse unconjugated monoclonal anti-CD31 IgG, and 1:50 dilution biotinylated rabbit anti-B. burgdorferi polyclonal IgG at 4°C overnight. On the following day, the samples were washed, and stained with 1:100 dilution of Alexa 555 goat anti-rat polyclonal IgG, and 1:200 dilution of Alexa 488 streptavidin for 1 hour at room temperature, covered from light. Secondary antibody-only controls for B. burgdorferi indirect fluorescent assay were performed in vitro and no fluorescence was observed.
Some of the dura samples were also stained for lymphatic vessels in a separate step, using 1:200 ab14917, followed by washing and secondary staining with 1:200 Alexa 633 goat-anti rabbit polyclonal IgG (yellow).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Image is courtesy of an anonymous Abreviewab14917 staining LYVE1 in mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-buffer pH 9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)This image is courtesy of an anonymous Abreviewab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (286)
ab14917 被引用在 286 文献中.
- Zhang PH et al. Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS. JCI Insight 9:N/A (2024). PubMed: 37971881
- Plá V et al. Structural characterization of SLYM-a 4th meningeal membrane. Fluids Barriers CNS 20:93 (2023). PubMed: 38098084
- Justynski O et al. Apoptosis recognition receptors regulate skin tissue repair in mice. Elife 12:N/A (2023). PubMed: 38127424
- Jung S et al. Clathrin light chain-conjugated drug delivery for cancer. Bioeng Transl Med 8:e10273 (2023). PubMed: 36684105
- Zhao P et al. Scar-Degrading Endothelial Cells as a Treatment for Advanced Liver Fibrosis. Adv Sci (Weinh) 10:e2203315 (2023). PubMed: 36494102