AB281056

重组Anti-LYST抗体[EPR23139-5] - BSA and Azide free (Detector)

Anti-LYST antibody [EPR23139-5] - BSA and Azide free (Detector)

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal LYST antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

CHS, CHS1, LYST, Lysosomal-trafficking regulator, Beige homolog

1 Images

Sandwich ELISA - Anti-LYST antibody [EPR23139-5] - BSA and Azide free (Detector) (AB281056)

sELISA

Supplier Data

Sandwich ELISA - Anti-LYST antibody [EPR23139-5] - BSA and Azide free (Detector) (AB281056)

Example of Human LYST standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23139-5

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

产品详情

ab281056 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LYST. The recommended pair for sandwich ELISA is:
Capture: ab281206, Human LYST Capture Antibody (unconjugated)
Detector: ab281056, Human LYST Detector Antibody (unconjugated)
The reference range value is 234 - 15000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The LYST protein also known as Lysosomal Trafficking Regulator or Beige is involved in intracellular transport processes. With an approximate molecular mass of 429 kDa it plays a role in the trafficking of lysosomal contents. Expressed in various tissues it particularly affects those with a high demand for lysosomal activity such as immune system cells. As part of its mechanical function LYST regulates the morphology of lysosomes and endosomes by facilitating fusion and movement of these vesicles.

Biological function summary

LYST directly influences the cellular degradation and recycling process. The protein does not act alone but functions as part of a larger cellular machinery that maintains lysosomal homeostasis. Its role is particularly important in immune cell function where it ensures proper lysosomal delivery and secretion especially in cytotoxic T cells and natural killer cells. These activities are important for granulocyte and lymphocyte regulation ensuring a proper response to infections and cellular stress.

Pathways

The protein LYST is integral in pathways related to vesicular transport such as the endocytic and autophagic pathways. It has a well-documented interaction with proteins such as Rab7 which is important for endosome-to-lysosome trafficking. Additionally LYST interacts with components of the cytoskeleton which further influences lysosome positioning within the cell therefore supporting its central role in cellular logistic pathways.

Mutations or functional disruptions of the LYST protein are linked to Chediak-Higashi Syndrome a rare genetic disorder characterized by immunodeficiency bleeding issues and neurological problems. This condition demonstrates a connection between LYST and the immune response as well as interactions with proteins like Perforin which are also involved in immune functions. Moreover LYST alterations might contribute to pigmentary anomalies in certain skin disorders due to its involvement in melanosome biogenesis.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes (PubMed : 11984006, PubMed : 25216107). Might regulate trafficking of effectors involved in exocytosis (PubMed : 25425525). In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules (PubMed : 26478006). In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes (By similarity). In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways (PubMed : 27881733).

See full target information LYST

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-LYST抗体[EPR23139-5] - BSA and Azide free (Detector)

Anti-LYST antibody [EPR23139-5] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-LYST antibody [EPR23139-5] - BSA and Azide free (Detector) (AB281056)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原