重组Anti-LysRS抗体[EPR7921] (ab129080)
![Western blot - Anti-LysRS antibody [EPR7921] (ab129080)](https://www.abcam.cn/ps/products/129/ab129080/Images/ab129080-143033-anti-lysrs-antibody-epr7921-western-blot.jpg "Western blot - Anti-LysRS antibody [EPR7921] (ab129080)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7921] to LysRS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LysRS抗体[EPR7921]
参阅全部 LysRS 一抗 -
描述
兔单克隆抗体[EPR7921] to LysRS -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human LysRS aa 550-650.
Database link: Q15046 -
阳性对照
- Jurkat, HeLa, 293T, HepG2, and Caco 2 cell lysates; Human colon tissue This antibody gave a positive result when used in the following methanol fixed cell lines: CACO-2
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.44 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7921 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129080于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 68 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/250. |
应用说明
Is unsuitable for Flow Cyt.
靶标
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功能
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. -
疾病相关
Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
序列相似性
Belongs to the class-II aminoacyl-tRNA synthetase family. -
结构域
The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2. -
细胞定位
Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha. - Information by UniProt
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数据库链接
- Entrez Gene: 3735 Human
- Entrez Gene: 85305 Mouse
- Entrez Gene: 292028 Rat
- Omim: 601421 Human
- SwissProt: Q15046 Human
- SwissProt: Q99MN1 Mouse
- Unigene: 3100 Human
- Unigene: 196544 Mouse
see all -
别名
- CMTRIB antibody
- DFNB89 antibody
- EC 6.1.1.6 antibody
see all
图片
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Western blot - Anti-LysRS antibody [EPR7921] (ab129080)All lanes : Anti-LysRS antibody [EPR7921] (ab129080) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] (ab129080)](https://www.abcam.cn/ps/products/129/ab129080/Images/ab129080-220067-anti-lysrs-antibody-epr7921-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] (ab129080)ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] (ab129080)](https://www.abcam.cn/ps/products/129/ab129080/Images/ab129080-143032-anti-lysrs-antibody-epr7921-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] (ab129080)ab129080, at a dilution of 1/50, staining LysRS in paraffin embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-LysRS antibody [EPR7921] (ab129080)](https://www.abcam.cn/ps/products/129/ab129080/Images/ab129080-143031-anti-lysrs-antibody-epr7921.jpg)
OI-RD Scanning - Anti-LysRS antibody [EPR7921] (ab129080) -
![Anti-LysRS antibody [EPR7921] (ab129080)](https://www.abcam.cn/ps/products/129/ab129080/Images/ab129080-6-benefits-of-recombinant-antibodies.png)
Anti-LysRS antibody [EPR7921] (ab129080)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab129080 被引用在 5 文献中.
- Jin D et al. HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit. Viruses 15:N/A (2023). PubMed: 36851687
- Sleigh JN et al. Altered Sensory Neuron Development in CMT2D Mice Is Site-Specific and Linked to Increased GlyRS Levels. Front Cell Neurosci 14:232 (2020). PubMed: 32848623
- Scheidecker S et al. Mutations in KARS cause a severe neurological and neurosensory disease with optic neuropathy. Hum Mutat 40:1826-1840 (2019). PubMed: 31116475
- Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . PubMed: 26544075
- Kim K et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One 8:e81734 (2013). WB, IP ; Human . PubMed: 24312579