AB253001

Anti-LYRM4抗体

Name: LYRM4抗体 (ab253001)| Abcam中文官网
Brand: Abcam Limited
SKU: AB253001

Anti-LYRM4 antibody

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(2 Publications)

Rabbit Polyclonal LYRM4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LYRM4 aa 1 to C-terminus.

查看别名

C6orf149, ISD11, CGI-203, LYRM4, LYR motif-containing protein 4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM4 antibody (AB253001)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM4 antibody (AB253001)

Paraffin-embedded human hippocampus tissue stained for LYRM4 using ab253001 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LYRM4 antibody (AB253001)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LYRM4 antibody (AB253001)

PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for LYRM4 (green) using ab253001 at 4 μg/ml in ICC/IF.

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Western blot - Anti-LYRM4 antibody (AB253001)

All lanes:

Western blot - Anti-LYRM4 antibody (ab253001) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

LYRM4 overexpression HEK-293T lysate

Predicted band size: 11 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human LYRM4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HD34

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LYRM4 also known as ISD11 is a mitochondrial protein with a mass of approximately 11 kDa. Mechanically LYRM4 plays a role in the regulation of mitochondrial metabolic activities specifically assisting in the maturation of the iron-sulfur (Fe-S) clusters important for respiratory function. LYRM4 is expressed in tissues with high mitochondrial activity such as muscle and liver. It forms a complex with the mitochondrial protein NFS1 enhancing its stability and functionality.

Biological function summary

LYRM4 associates with processes vital for cell metabolic homoeostasis. It forms part of the mitochondrial iron-sulfur cluster assembly machinery a complex essential for synthesizing Fe-S clusters. These clusters are required for numerous cellular functions including electron transport and enzyme activities. LYRM4 works closely with other components like ISCU and FXN helping to provide functional clusters to mitochondrial enzymes.

Pathways

LYRM4 functions within the context of mitochondrial iron-sulfur cluster biosynthesis and electron transport chain pathways. These pathways are critical for energy production and cellular metabolism. LYRM4 relates to proteins such as NFS1 and ISCU through these pathways facilitating the proper assembly and delivery of Fe-S clusters needed to maintain oxidative phosphorylation and cellular respiration.

LYRM4 mutations or dysfunctions relate to conditions such as mitochondrial myopathy and Friedreich's ataxia. These disorders involve impaired mitochondrial function and oxidative stress which stem from deficiencies in Fe-S cluster formation and function. The protein’s interactions with others like FXN implicated in Friedreich's ataxia highlight its importance in mitochondrial health and disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Stabilizing factor, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with NDUFAB1, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed : 17331979, PubMed : 31664822). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). May also participates in the iron-sulfur protein biogenesis in the cytoplasm through its interaction with the cytoplasmic form of NFS1 (PubMed : 19454487).

See full target information LYRM4

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Orphanet journal of rare diseases 20:453 PubMed40849475

2025

Alpha-lipoic acid supplementation improves pathological alterations in cellular models of Friedreich ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Talaverón-Rey,Diana Reche-López,Suleva Povea-Cabello,Mónica Álvarez-Córdoba,David Gómez-Fernández,Ana Romero-González,Paula Cilleros-Holgado,José Manuel Romero-Domínguez,Alejandra López-Cabrera,Rocío Piñero-Pérez,Susana González-Granero,José Manuel García-Verdugo,José A Sánchez-Alcázar

Antioxidants (Basel, Switzerland) 14: PubMed40002401

2025

Polydatin and Nicotinamide Prevent Iron Accumulation and Lipid Peroxidation in Cellular Models of Mitochondrial Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero-Domínguez,Diana Reche-López,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristán,Susana González-Granero,José Manuel García-Verdugo,José Antonio Sánchez-Alcázar

View all publications

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Anti-LYRM4抗体

Anti-LYRM4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM4 antibody (AB253001)

Immunocytochemistry/ Immunofluorescence - Anti-LYRM4 antibody (AB253001)

Western blot - Anti-LYRM4 antibody (AB253001)