重组Anti-LYRIC/AEG1抗体[EP4445] (ab124789)
![Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)](https://www.abcam.cn/ps/products/124/ab124789/Images/ab124789-138673-anti-lyric-antibody-ep4445-western-blot.jpg "Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4445] to LYRIC/AEG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-LYRIC/AEG1抗体[EP4445]
参阅全部 LYRIC/AEG1 一抗 -
描述
兔单克隆抗体[EP4445] to LYRIC/AEG1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human LYRIC/AEG1 aa 250-350. The exact sequence is proprietary.
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阳性对照
- HeLa, Jurkat, PC3, PC12 and NIH 3T3 cell lysates; Human brain and breast tissues; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP4445 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124789于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
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| ICC/IF |
1/500.
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| 说明 |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
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ICC/IF
1/500. |
靶标
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功能
Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. -
组织特异性
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. -
细胞定位
Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 92140 Human
- Entrez Gene: 67154 Mouse
- Entrez Gene: 170910 Rat
- Omim: 610323 Human
- SwissProt: Q86UE4 Human
- SwissProt: Q80WJ7 Mouse
- SwissProt: Q9Z1W6 Rat
- Unigene: 377155 Human
see all -
别名
- 3D3 antibody
- 3D3/LYRIC antibody
- AEG 1 antibody
see all
图片
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Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)All lanes : Anti-LYRIC/AEG1 antibody [EP4445] (ab124789) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)](https://www.abcam.cn/ps/products/124/ab124789/Images/ab124789-138671-anti-lyric-antibody-ep4445-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)](https://www.abcam.cn/ps/products/124/ab124789/Images/ab124789-266091-anti-lyric-antibody-ep4445-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control: PBS only.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)](https://www.abcam.cn/ps/products/124/ab124789/Images/ab124789-138672-anti-lyric-antibody-ep4445-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)](https://www.abcam.cn/ps/products/124/ab124789/Images/ab124789-6-benefits-of-recombinant-antibodies.png)
Anti-LYRIC/AEG1 antibody [EP4445] (ab124789)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (16)
ab124789 被引用在 16 文献中.
- Ortiz-Soto G et al. Metadherin Regulates Inflammatory Breast Cancer Invasion and Metastasis. Int J Mol Sci 24:N/A (2023). PubMed: 36902125
- Peng KL et al. Miat and interacting protein Metadherin maintain a stem-like niche to promote medulloblastoma tumorigenesis and treatment resistance. Proc Natl Acad Sci U S A 119:e2203738119 (2022). PubMed: 36067288
- Wei W et al. LOC102553417 silencing facilitates the apoptosis of hepatic stellate cells via the miR‑30e/MTDH axis. Mol Med Rep 26:N/A (2022). PubMed: 36177898
- Deng J et al. Long Noncoding RNA DLGAP1-AS1 Promotes the Aggressive Behavior of Gastric Cancer by Acting as a ceRNA for microRNA-628-5p and Raising Astrocyte Elevated Gene 1 Expression. Cancer Manag Res 12:2947-2960 (2020). PubMed: 32431541
- Fan H et al. Long Non-Coding RNA DARS-AS1 Contributes to Prostate Cancer Progression Through Regulating the MicroRNA-628-5p/MTDH Axis. Cancer Manag Res 12:8363-8377 (2020). PubMed: 32982430