重组Anti-LYPD3抗体[SP250] - BSA and Azide free (ab271958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP250] to LYPD3 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LYPD3抗体[SP250] - BSA and Azide free
参阅全部 LYPD3 一抗 -
描述
兔单克隆抗体[SP250] to LYPD3 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human skin tissue. Flow Cytometry: BxPC-3 cells.
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常规说明
ab271958 is the carrier-free version of ab192845.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by Protein A/G. -
克隆
单克隆 -
克隆编号
SP250 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-LYPD3 antibody [SP250] - N-terminal (ab192845)
- Alexa Fluor® 488 Anti-LYPD3 antibody [SP250] - N-terminal (ab309938)
- Alexa Fluor® 647 Anti-LYPD3 antibody [SP250] - N-terminal (ab310305)
- Alexa Fluor® 594 Anti-LYPD3 antibody [SP250] - N-terminal (ab310768)
- Alexa Fluor® 555 Anti-LYPD3 antibody [SP250] - N-terminal (ab312295)
- Alexa Fluor® 568 Anti-LYPD3 antibody [SP250] - N-terminal (ab312790)
- Alexa Fluor® 750 Anti-LYPD3 antibody [SP250] - N-terminal (ab321327)
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271958于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature
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|
Flow Cyt |
Use at an assay dependent concentration.
Incubate with primary antibody for 30 minutes at 4°C.
|
说明 |
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IHC-P
Use at an assay dependent concentration. Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature
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Flow Cyt
Use at an assay dependent concentration. Incubate with primary antibody for 30 minutes at 4°C.
|
靶标
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功能
Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. -
组织特异性
Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. -
序列相似性
Contains 2 UPAR/Ly6 domains. -
翻译后修饰
N-glycosylated and O-glycosylated. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 27076 Human
- Entrez Gene: 72434 Mouse
- Entrez Gene: 60378 Rat
- Omim: 609484 Human
- SwissProt: O95274 Human
- SwissProt: Q91YK8 Mouse
- SwissProt: O55162 Rat
- Unigene: 631594 Human
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别名
- 2310061G07Rik antibody
- C4.4A antibody
- GPI-anchored metastasis-associated protein C4.4A homolog antibody
see all
图片
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Flow cytometric analysis of BxPC-3 (human pancreas adenocarcinoma cell line) cells labeling LYPD3 with ab192845 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192845).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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