Anti-Lyn抗体[LYN-01] (ab1890)
![Western blot - Anti-Lyn antibody [LYN-01] (ab1890)](https://www.abcam.cn/ps/products/1/ab1890/Images/ab1890-455395-anti-lyn-antibody-western-blot-cell-lines-human-rat.jpg "Western blot - Anti-Lyn antibody [LYN-01] (ab1890)")
Key features and details
- Mouse monoclonal [LYN-01] to Lyn
- Suitable for: Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Lyn抗体[LYN-01]
参阅全部 Lyn 一抗 -
描述
小鼠单克隆抗体[LYN-01] to Lyn -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment corresponding to Human Lyn aa 1-250.
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常规说明
Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Primary antibody说明
Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI). -
克隆
单克隆 -
克隆编号
LYN-01 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | (1) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| WB | (3) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa.
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| 说明 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 58 kDa. |
靶标
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功能
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
组织特异性
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
结构域
The protein kinase domain plays an important role in its localization in the cell membrane. -
翻译后修饰
Ubiquitinated. Ubiquitination is SH3-dependent. -
细胞定位
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
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数据库链接
- Entrez Gene: 4067 Human
- Entrez Gene: 81515 Rat
- Omim: 165120 Human
- SwissProt: P07948 Human
- SwissProt: Q07014 Rat
- Unigene: 491767 Human
- Unigene: 4338 Rat
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形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- AA407514 antibody
- EC 2.7.10.2 antibody
- FLJ26625 antibody
see all
图片
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Western blot - Anti-Lyn antibody [LYN-01] (ab1890)All lanes : Anti-Lyn antibody [LYN-01] (ab1890) at 1 µg/ml
Lane 1 : Negative control
Lane 2 : Rat basophilic leukemia cell line
Lane 3 : JURKAT human peripheral blood T cell leukemia cell line
Lane 4 : A-431 human epidermoid carcinoma cell line
Lane 5 : U-937 human histiocytic lymphoma cell line
Predicted band size: 58 kDaNon reducing conditions with 60 minutes exposure time in a 12% gel
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![Western blot - Anti-Lyn antibody [LYN-01] (ab1890)](https://www.abcam.cn/ps/products/1/ab1890/Images/Lyn-Primary-antibodies-ab1890-11.jpg)
Western blot - Anti-Lyn antibody [LYN-01] (ab1890)Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + Recombinant human Lyn protein (ab60306) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 4 minutes -
![Western blot - Anti-Lyn antibody [LYN-01] (ab1890)](https://www.abcam.cn/ps/products/1/ab1890/Images/Lyn-Primary-antibodies-ab1890-6.jpg)
Western blot - Anti-Lyn antibody [LYN-01] (ab1890)Image courtesy of an anonymous Abreview.Anti-Lyn antibody [LYN-01] (ab1890) at 1/1000 dilution + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 50 µg
Secondary
HRP-conjugated anti-mouse at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-Lyn antibody [LYN-01] (ab1890)](https://www.abcam.cn/ps/products/1/ab1890/Images/Lyn-Primary-antibodies-ab1890-17.jpg)
Flow Cytometry - Anti-Lyn antibody [LYN-01] (ab1890)Overlay histogram showing Ramos (Human Burkitt's lymphoma cell line) cells stained with ab1890 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1890, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab1890 被引用在 17 文献中.
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Brian BF et al. Unique-region phosphorylation targets LynA for rapid degradation, tuning its expression and signaling in myeloid cells. Elife 8:N/A (2019). PubMed: 31282857
- Gavali S et al. Estrogen enhances human osteoblast survival and function via promotion of autophagy. Biochim Biophys Acta Mol Cell Res 1866:1498-1507 (2019). PubMed: 31255720
- Lin J et al. Characterizing the molecular mechanisms of acquired temozolomide resistance in the U251 glioblastoma cell line by protein microarray. Oncol Rep 39:2333-2341 (2018). PubMed: 29565460
- Tornillo G et al. Dual Mechanisms of LYN Kinase Dysregulation Drive Aggressive Behavior in Breast Cancer Cells. Cell Rep 25:3674-3692.e10 (2018). PubMed: 30590041