AB182138

重组Anti-LYAR抗体[EPR14352-9]

Anti-LYAR antibody [EPR14352-9]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal LYAR antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

PNAS-5, LYAR, Cell growth-regulating nucleolar protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (AB182138)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352-9] (AB182138)

Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling LYAR with ab182138 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (AB182138)

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab182138 at 1/60 dilution (Lane 1). Lane 2 : Negative control.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-LYAR antibody [EPR14352-9] (ab182138)

Predicted band size: 44 kDa

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Supplier Data

Western blot - Anti-LYAR antibody [EPR14352-9] (AB182138)

All lanes:

Western blot - Anti-LYAR antibody [EPR14352-9] (ab182138) at 1/20000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-LYAR antibody [EPR14352-9] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14352-9

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Lyar also known as the cell growth-regulating nucleolar protein is a zinc finger protein with a molecular mass of approximately 36 kDa. This protein is expressed primarily in the nucleolus although it can also be found in other sub-nuclear structures. Lyar binds to nucleic acids and proteins playing roles in nucleolus-related processes. Its expression is seen in various tissues but it is most pronounced in rapidly dividing cells such as those in fetal tissues and certain cancers.

Biological function summary

The Lyar protein plays a role in regulating cell growth and proliferation. It participates in ribosome biogenesis by interacting with pre-ribosomal RNA possibly aiding in the stabilization and processing of rRNA precursors. Lyar is not usually considered as part of a protein complex but its interactions with several other nucleolar proteins imply its involvement in complex regulatory networks. It influences the transcription of ribosomal RNA genes impacting protein synthesis rates and cell cycle progression.

Pathways

Lyar is involved in the modulation of pathways linked to cell proliferation and survival. Notably it affects the PI3K/AKT signaling pathway by interacting with nucleophosmin (NPM1) impacting cell growth and survival mechanisms. This collaboration can alter the response of cells to external growth signals influencing cellular responses to metabolic changes and stress.

Aberrant Lyar levels have been implicated in oncogenesis. Its overexpression has been noted in various cancers including leukemia and neuroblastoma linking it to uncontrolled cell division and tumor progression. In cancer Lyar interacts with tumorigenesis-associated proteins such as c-Myc enhancing their transcriptional activity and contributing to cancerous transformation. Understanding the role of Lyar in these disease processes remains essential for identifying potential therapeutic strategies and developing targeted interventions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed : 24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed : 25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed : 28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed : 25092918). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed : 31413131). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed : 31413131). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).

See full target information LYAR

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8870 PubMed41053178

2025

Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve-neutrophil axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Tao Liu,Yang Wang,Xue-Mei Miao,Yi-Yu Xiong,Qian Zhao,Wei-Yun Shen,Fu-Hong Su,Kang Chen,Ru-Ping Dai

Cancer discovery 13:332-347 PubMed36259929

2022

A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Fengbiao Zhou,Nesrine Aroua,Yi Liu,Christian Rohde,Jingdong Cheng,Anna-Katharina Wirth,Daria Fijalkowska,Stefanie Göllner,Michelle Lotze,Haiyang Yun,Xiaobing Yu,Caroline Pabst,Tim Sauer,Thomas Oellerich,Hubert Serve,Christoph Röllig,Martin Bornhäuser,Christian Thiede,Claudia Baldus,Michaela Frye,Simon Raffel,Jeroen Krijgsveld,Irmela Jeremias,Roland Beckmann,Andreas Trumpp,Carsten Müller-Tidow

View all publications

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重组Anti-LYAR抗体[EPR14352-9]