Anti-Ly6g抗体[1A8] - Low endotoxin，Azide free

• Flow Cyt

Lab

Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (AB210204)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab320706 (right) or Rat IgG2a, kappa monoclonal (ab18450), isotype control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10% mouse serum and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab320706 or Rat IgG2a, kappa monoclonal (ab18450), isotype control (1x 106 in 100 μl at 1.0 μg/ml ) for 30min on ice. The cells were simultaneously stained with APC anti-mouse Ly-6C Antibody.

The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

• FuncS (Depl)

Collaborator

Functional Studies (Depl) - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (AB210204)

Flow cytometric analysis of peripheral blood samples from neutrophil Ly6G-Tomato reporter mice 48h post intraperitoneal injection with 500μg of anti-Ly6G [1A8] antibody mouse IgG2c (chimeric) (ab320708) (A) or Anti-Ly6g [1A8] antibody – rat IgG2a (ab210204) (B) and corresponding isotype controls.

ab320708 injections result in prolonged neutrophils depletion compared to ab210204.

Cells were stained with CD45 conjugated to PE-Cy7 and CD11b conjugated to APC- eFluor 780. Gated on viable CD45+ population for analysis of CD11+ Ly6G-tdTomato+ neutrophils.

Developed in collaboration with Tatyana Chtanova lab, Garvan Institute of Medical Research and UNSW Sydney, Australia.