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重组RabMAb

重组Anti-LY6C抗体[RM1151] - BSA and Azide free (ab317273)

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Western blot - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Multiplex immunohistochemistry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Immunoprecipitation - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
  • Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RM1151] to Ly6c - BSA and Azide free
  • Suitable for: mIHC, Flow Cyt, WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-LY6C抗体[RM1151] - BSA and Azide free
    参阅全部 Ly6c 一抗

  • 描述

    兔重组multiclonal [RM1151] to Ly6c - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: mIHC, Flow Cyt, WB, IHC-P, ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse
    不与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse lung, Mouse spleen, Mouse testis, Mouse kidney, NIH/3T3 and C2C12 lysates. IHC-P: Mouse spleen, Mouse colon and Mouse cerebrum tissues. MIHC: Mouse spleen tissue. ICC/IF: Mouse bone marrow and Mouse splenocyte cells. Flow Cyt: Mouse bone marrow and Mouse spleen cells. IP: Mouse spleen tissue lysate.

  • 常规说明

    ab317273 is the carrier-free version of ab317272.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317273于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
mIHC
Use at an assay dependent concentration.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
mIHC
Use at an assay dependent concentration.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

靶标

  • 相关性

    Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.

  • 细胞定位

    Cell membrane; Lipid-anchor, GPI-anchor.

  • 数据库链接

    • 别名

      • Ly 6c antibody
      • Ly6c protein antibody
      • Ly6c1 antibody
      • Ly6c2 antibody
      • Lymphocyte antigen 6 complex antibody
      • Lymphocyte antigen 6 complex locus C antibody
      • Lymphocyte antigen 6C antibody
      • Lymphocyte antigen 6C1 antibody
      • Lymphocyte antigen 6C2 antibody
      • Lymphocyte antigen Ly 6C antibody
      see all

    图片

    • Western blot - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Western blot - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      All lanes : Anti-LY6C antibody [RM1151] (ab317272) at 1/1000 dilution

      Lane 1 : Mouse lung tissue lysate
      Lane 2 : Mouse spleen tissue lysate
      Lane 3 : Mouse testis tissue lysate
      Lane 4 : Mouse liver tissue lysate
      Lane 5 : Mouse kidney tissue lysate
      Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
      Lane 7 : C2C12 (mouse myoblast) whole cell lysate
      Lane 8 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 14 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: Neuro-2a.

      Low expression: testis, liver.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on endothelial cells and immune cells in mouse spleen. The section was incubated with ab317272 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on endothelial cells and immune cells in mouse colon. The section was incubated with ab317272 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on endothelial cells in mouse cerebrum. The section was incubated with ab317272 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression tissue: no staining on mouse liver. The section was incubated with ab317272 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Multiplex immunohistochemistry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Multiplex immunohistochemistry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272 the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen labelling LY6C with ab317272 at 1/100 (B), CD11b with ab133357 at 1/20000 dilution (C) and CD19 with ab245235 at 1/1000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Panel A: merged staining of anti-LY6C (green; Opal™690), anti-CD11b (magenta; Opal™570) and anti-CD19 (yellow; Opal™520) on mouse spleen.
      Panel B: anti-LY6C stained on monocytes/macrophages.
      Panel C: anti-CD11b stained on monocytes/macrophages.
      Panel D: anti-CD19 stained on B cells.
      Co-staining of LY6C and CD11b can be observed.

      The section was incubated in three rounds of staining: in the order of ab317272, ab133357, and ab245235 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    • Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

      Confocal image showing cytoplasmic staining in subsets of mouse bone marrow (shown in green), which is partially co-located with CD11b. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

      Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

    • Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse bone marrow cells labelling LY6C with ab317272 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Mouse bone marrow are co-stained with CD11b conjugated BV510 (512/25BP).
      Gated on viable cell.

    • Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse bone marrow cells labelling LY6C with ab317272 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Mouse bone marrow are co-stained with B220 conjugated Alexa Fluor®647.
      Gated on viable cell.

    • Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Flow Cytometry - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Mouse spleen cell cells labelling LY6C with ab317272 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Mouse spleen cell are co-stained with CD8a conjugated Alexa Fluor®647.
      Gated on viable cell.

    • Immunoprecipitation - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Immunoprecipitation - Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

      This data was developed using ab317272, the same antibody clone in a different buffer formulation.

      LY6C was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab317272 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317272 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse spleen tissue lysate
      Lane 2: ab317272 IP in Mouse spleen tissue lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317272 in mouse spleen tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 128 seconds.

    • Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)
      Anti-LY6C antibody [RM1151] - BSA and Azide free (ab317273)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab317273 尚未被引用在任何文献中。

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