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重组RabMAb

重组Anti-Ly-6A/E (Sca-1)抗体[EPR3355] (ab109211)

Reviews (1)Q&A (1)References (11)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
  • Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
  • Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
  • Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3355] to Ly-6A/E (Sca-1)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-Ly-6A/E (Sca-1)抗体[EPR3355]
    参阅全部 Ly-6A/E (Sca-1) 一抗

  • 描述

    兔单克隆抗体[EPR3355] to Ly-6A/E (Sca-1)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse bone marrow, and Hepa1-6 lysates. IHC-P: mouse kidney tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109211于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P (1)
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended. See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100
说明
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended. See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

  • 功能

    T-cell activation.

  • 组织特异性

    Widely expressed.

  • 序列相似性

    Contains 1 UPAR/Ly6 domain.

  • 翻译后修饰

    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Ly6A/E (Sca1) with Purified ab109211 at 1:150 dilution (1.37 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211) at 1/10000 dilution (Purified) + Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211) at 1/1000 dilution (Purified) + Mouse bone marrow lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)

      ab109211, at 1/50 dilution staining Ly6A/E (Sca1) in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)Image from Basile LA et al., PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24, 2012, PLoS ONE 7(2): e30434.

      Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)
      Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211)

    文献 (11)

    发表研究结果有使用 ab109211？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109211 被引用在 11 文献中.

    • Bala P  et al. Aberrant cell state plasticity mediated by developmental reprogramming precedes colorectal cancer initiation. Sci Adv 9:eadf0927 (2023). PubMed: 36989360
    • Xie BS  et al. Apolipoprotein E, low-density lipoprotein receptor, and immune cells control blood-brain barrier penetration by AAV-PHP.eB in mice. Theranostics 11:1177-1191 (2021). PubMed: 33391529
    • Zhu D  et al. Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair. Nat Commun 12:1412 (2021). PubMed: 33658506
    • Batista AR  et al. Ly6a Differential Expression in Blood-Brain Barrier Is Responsible for Strain Specific Central Nervous System Transduction Profile of AAV-PHP.B. Hum Gene Ther 31:90-102 (2020). PubMed: 31696742
    • Silva-Pinheiro P  et al. A Single Intravenous Injection of AAV-PHP.B-hNDUFS4 Ameliorates the Phenotype of Ndufs4-/- Mice. Mol Ther Methods Clin Dev 17:1071-1078 (2020). PubMed: 32478122
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    Yes - Tween-20
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Reshef Tal

    Verified customer

    提交于 Sep 04 2019

    Question

    Were denaturing and reducing WB conditions used? 

    Read More
    Answer

    Thank you for contacting us. I have heard back from the lab: For WB, denaturing and reducing conditions were used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

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