AB239951

重组Anti-Ly-6A/E (Sca-1)抗体[EPR3355] - BSA and Azide free

Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Sca1 / Ly6A/E antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 1 publication.

查看别名

Ly6, Ly6a, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, T-cell-activating protein, SCA-1, TAP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free (AB239951)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free (AB239951)

ab109211, at 1/50 dilution staining Ly6A/E (Sca1) in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109211).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free (AB239951)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Ly6A/E (Sca1) with Purified ab109211 at 1 : 150 dilution (1.37 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109211)

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free (AB239951)

Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Ly6A/E (Sca1) with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109211).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Image from Basile LA et al., PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24, 2012, PLoS ONE 7(2): e30434.

不同偶联物与剂型 (8)

Unconjugated

Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
660 APC

APC Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]
578 PE

PE Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3355

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Antigen retrieval is recommended.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

style

left-column

产品详情

ab239951 is the carrier-free version of ab109211.

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

T-cell activation.

See full target information Ly6a

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 63: PubMed37449524

2023

circRPS19 affects HK2‑mediated aerobic glycolysis and cell viability via the miR‑125a‑5p/USP7 pathway in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Zheng,Jie Shao,Jun Qian,Shenlin Liu

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Ly-6A/E (Sca-1)抗体[EPR3355] - BSA and Azide free