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重组RabMAb

重组Anti-LXR alpha抗体[EPR6508(N)] (ab176323)

Reviews (2) Submit a question References (57)
Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
  • Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6508(N)] to LXR alpha
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

概述

  • 产品名称

    Anti-LXR alpha抗体[EPR6508(N)]
    参阅全部 LXR alpha 一抗

  • 描述

    兔单克隆抗体[EPR6508(N)] to LXR alpha

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Sheep

  • 免疫原

    Synthetic peptide within Human LXR alpha aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q13133
    (Peptide available as ab191835)

  • 阳性对照

    • WB: Jurkat, SH-SY5Y, IMR-32 cell non-nuclear and nuclear fractions, HEK 293 transfected with blank vectors, blank vectors non-nuclear fraction and blank vectors nuclear fraction, HEK 293 transfected with His tagged human LXR alpha full length expression vectors non-nuclear fraction and nuclear fraction, HEK 293 transfected with His tagged human LXR alpha and beta full length expression vectors.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176323于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
说明
WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
应用说明
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.

靶标

  • 功能

    Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

  • 组织特异性

    Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.

  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Liver X receptor alpha antibody
    • LXR a antibody
    • LXRA antibody
    • NR1H3 antibody
    • NR1H3_HUMAN antibody
    • Nuclear receptor subfamily 1 group H member 3 antibody
    • Oxysterols receptor LXR alpha antibody
    • Oxysterols receptor LXR-alpha antibody
    • RLD 1 antibody
    • RLD1 antibody
    see all

图片

  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) cells non-nuclear fraction
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) cells nuclear fraction
    Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) cells non-nuclear fraction
    Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) cells nuclear fraction
    Lane 5 : IMR-32 (Human neuroblastoma neuroblast) cells non-nuclear fraction
    Lane 6 : IMR-32 (Human neuroblastoma neuroblast) cells nuclear fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 7 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.

    ab176842 and ab181602 were used as loading controls.

  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

    Lane 1 : HEK 293 transfected with blank vectors, whole cell lysate
    Lane 2 : HEK 293 transfected with blank vectors, non-nuclear fraction
    Lane 3 : HEK 293 transfected with blank vectors, nuclear fraction
    Lane 4 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, non-nuclear fraction
    Lane 5 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, nuclear fraction

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 10 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.

    ab213204, ab176842 and ab181602 were used as loading controls.

  • Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    All lanes : Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

    Lane 1 : HEK 293 transfected with blank vectors, whole cell lysate
    Lane 2 : HEK 293 transfected with His tagged human LXR alpha full length expression vectors, whole cell lysate
    Lane 3 : HEK 293 transfected with His tagged human LXR beta full length expression vectors, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 seconds


    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Ab176323 does not cross activate with human LXR beta.

    ab213204 was used as loading control.

  • Anti-LXR alpha antibody [EPR6508(N)] (ab176323)
    Anti-LXR alpha antibody [EPR6508(N)] (ab176323)

文献 (57)

发表研究结果有使用 ab176323？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab176323 被引用在 57 文献中.

  • Hori M  et al. Acute Cholesterol-Lowering Effect of Exendin-4 in Ldlr-/- and C57BL/6J Mice. J Atheroscler Thromb 30:74-86 (2023). PubMed: 35314564
  • Kim YJ  et al. Impact of Ring Finger Protein 20 and Its Downstream Regulation on Renal Tubular Injury in a Unilateral Nephrectomy Mouse Model Fed a High-Fat Diet. Nutrients 15:N/A (2023). PubMed: 38068817
  • Liu J  et al. Construction of humanized CYP1A2 rats using CRISPR/Cas9 to promote drug metabolism and pharmacokinetic research. Drug Metab Dispos N/A:N/A (2023). PubMed: 37884392
  • Wang Y  et al. Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration. Life Sci Alliance 6:N/A (2023). PubMed: 37550000
  • Lin KH  et al. E2F7 drives autotaxin/Enpp2 transcription via chromosome looping: Repression by p53 in murine but not in human carcinomas. FASEB J 37:e23058 (2023). PubMed: 37358838
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-LXR alpha antibody [EPR6508(N)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Synovial membrane)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Synovial membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 09 2016

Western blot abreview for Anti-LXR alpha antibody [EPR6508(N)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SCC61)
Gel Running Conditions
Reduced Denaturing
Loading amount
23 µg
Specification
SCC61
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 15 2015

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