Anti-LXR alpha抗体(ab106464)
Key features and details
- Rabbit polyclonal to LXR alpha
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-LXR alpha抗体
参阅全部 LXR alpha 一抗 -
描述
兔多克隆抗体to LXR alpha -
宿主
Rabbit -
特异性
At leastthree isoforms of LXR alpha are known to exist; ab106464 will recognize all three. ab106464 is predicted to not crossreact with LXR beta. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to 15 amino acids from near the N terminal end of Human LXR alpha (NP_005684).
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阳性对照
- Rat liver tissue and tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab106464 was purified by affinity chromatography via a peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106464于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. -
组织特异性
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 58852 Rat
- SwissProt: Q62685 Rat
- Unigene: 11209 Rat
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别名
- Liver X receptor alpha antibody
- LXR a antibody
- LXRA antibody
see all
图片
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Western blot - Anti-LXR alpha antibody (ab106464)Lane 1 : Anti-LXR alpha antibody (ab106464) at 1 µg/ml
Lane 2 : Anti-LXR alpha antibody (ab106464) at 2 µg/ml
All lanes : Rat liver tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 50 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-LXR alpha antibody (ab106464)Immunofluorescence of LXR alpha in rat liver tissue with ab106464 at 20 ug/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody (ab106464)Immunohistochemistry of rat liver tissue with ab106464 at 5 μg/mL
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab106464 被引用在 8 文献中.
- Huang Q et al. Beneficial effects of Panax notoginseng (Burkill) F. H. Chen flower saponins in rats with metabolic hypertension by inhibiting the activation of the renin-angiotensin-aldosterone system through complement 3. BMC Complement Med Ther 23:13 (2023). PubMed: 36653797
- Fang F et al. Expression of NR1H3 in endometrial carcinoma and its effect on the proliferation of Ishikawa cells in vitro. Onco Targets Ther 12:685-697 (2019). PubMed: 30705597
- Grinman DY et al. Liver X receptor-a activation enhances cholesterol secretion in lactating mammary epithelium. Am J Physiol Endocrinol Metab 316:E1136-E1145 (2019). PubMed: 30964702
- Hu J et al. Silencing ZAP70 prevents HSP65-induced reverse cholesterol transport and NF-?B activation in T cells. Biomed Pharmacother 102:271-277 (2018). PubMed: 29567540
- Bakry OA et al. Immunohistochemical Expression of Cyclo-oxygenase 2 and Liver X Receptor-a in Acne Vulgaris. J Clin Diagn Res 11:WC01-WC07 (2017). PubMed: 29207817
- Luo T et al. Lck Inhibits Heat Shock Protein 65-Mediated Reverse Cholesterol Transport in T Cells. J Immunol 197:3861-3870 (2016). PubMed: 27742830
- Pytlowanciv EZ et al. Differential ontogenetic exposure to obesogenic environment induces hyperproliferative status and nuclear receptors imbalance in the rat prostate at adulthood. Prostate 76:662-78 (2016). PubMed: 26847797
- Miroshnikova VV et al. [Regulation of ABCA1 and ABCG1 gene expression in the intraabdominal adipose tissue]. Biomed Khim 62:283-9 (2016). PubMed: 27420620