重组Anti-LTK抗体[EPR5175(2)] (ab129155)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5175(2)] to LTK
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LTK抗体[EPR5175(2)]
参阅全部 LTK 一抗 -
描述
兔单克隆抗体[EPR5175(2)] to LTK -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human LTK aa 800-900 (C terminal). The exact sequence is proprietary.
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阳性对照
- Raji, Human tonsil and Human spleen lysates; Human colon tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 7.60 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5175(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129155于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 92 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Orphan receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport. -
组织特异性
Expressed in non-hematopoietic cell lines and T- and B-cell lines. -
疾病相关
Note=Genetic variations in LTK that cause up-regulation of the PI3K pathway may possibly contribute to susceptibility to abnormal proliferation of self-reactive B cells and, therefore, to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue, thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4058 Human
- Omim: 151520 Human
- SwissProt: P29376 Human
- Unigene: 434481 Human
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别名
- Leukocyte receptor tyrosine kinase antibody
- Leukocyte tyrosine kinase antibody
- Leukocyte tyrosine kinase receptor antibody
see all
图片
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All lanes : Anti-LTK antibody [EPR5175(2)] (ab129155) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTK antibody [EPR5175(2)] (ab129155)
ab129155, at a 1/250 dilution, staining LTK in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab129155 被引用在 1 文献中.
- Izumi H et al. The CLIP1-LTK fusion is an oncogenic driver in non-small-cell lung cancer. Nature 600:319-323 (2021). PubMed: 34819663