Anti-LTBR抗体(ab226377)
Key features and details
- Rabbit polyclonal to LTBR
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LTBR抗体
参阅全部 LTBR 一抗 -
描述
兔多克隆抗体to LTBR -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human LTBR aa 385-435. The exact sequence is proprietary.
Database link: P36941 -
阳性对照
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226377 was affinity purified using an epitope specific to LTBR immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226377于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
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| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
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WB
1/1000 - 1/5000. Predicted molecular weight: 47 kDa. |
靶标
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功能
Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT. Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs. -
序列相似性
Contains 4 TNFR-Cys repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 451778 Chimpanzee
- Entrez Gene: 101149920 Gorilla
- Entrez Gene: 4055 Human
- Entrez Gene: 100171849 Orangutan
- Omim: 600979 Human
- SwissProt: P36941 Human
- Unigene: 1116 Human
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别名
- CD18 antibody
- D12S370 antibody
- LT beta R antibody
see all
图片
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Western blot - Anti-LTBR antibody (ab226377)All lanes : Anti-LTBR antibody (ab226377) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-LTBR antibody (ab226377)LTBR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226377 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226377 at 0.4 µg/ml.
Lane 1: ab226377 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226377 尚未被引用在任何文献中。