重组Anti-LSS抗体[EPR6704(B)] (ab124785)
![Western blot - Anti-LSS antibody [EPR6704(B)] (ab124785)](https://www.abcam.cn/ps/products/124/ab124785/Images/ab124785-138665-anti-lss-antibody-epr6704b-western-blot.jpg "Western blot - Anti-LSS antibody [EPR6704(B)] (ab124785)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6704(B)] to LSS
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-LSS抗体[EPR6704(B)]
参阅全部 LSS 一抗 -
描述
兔单克隆抗体[EPR6704(B)] to LSS -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human LSS aa 650-750. The exact sequence is proprietary.
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阳性对照
- WB: SH-SY5Y, HeLa, HT-1376 and Human fetal liver lysates. IHC-P: Human kidney tissue. Flow Cyt (intra): HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
解离常数（KD）
KD = 6.20 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6704(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124785于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000 - 1/200000. Predicted molecular weight: 83 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| 说明 |
|---|
|
WB
1/50000 - 1/200000. Predicted molecular weight: 83 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
靶标
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相关性
Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. -
数据库链接
- Entrez Gene: 4047 Human
- Omim: 600909 Human
- SwissProt: P48449 Human
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别名
- 2,3 epoxysqualene lanosterol cyclase antibody
- hOSC antibody
- Lanosterol synthase (2,3 oxidosqualene lanosterol cyclase) antibody
see all
图片
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Western blot - Anti-LSS antibody [EPR6704(B)] (ab124785)All lanes : Anti-LSS antibody [EPR6704(B)] (ab124785) at 1/50000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT-1376 cell lysate
Lane 4 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6704(B)] (ab124785)](https://www.abcam.cn/ps/products/124/ab124785/Images/ab124785-138664-anti-lss-antibody-epr6704b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6704(B)] (ab124785)ab124785 at 1/50 dilution staining LSS in paraffin-embedded Human kidney tissues by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-LSS antibody [EPR6704(B)] (ab124785)](https://www.abcam.cn/ps/products/124/ab124785/Images/ab124785-5-anti-lss-antibody-epr6704-b-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-LSS antibody [EPR6704(B)] (ab124785)Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling LSS with purified ab124785 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![OI-RD Scanning - Anti-LSS antibody [EPR6704(B)] (ab124785)](https://www.abcam.cn/ps/products/124/ab124785/Images/ab124785-138663-anti-lss-antibody-epr6704b.jpg)
OI-RD Scanning - Anti-LSS antibody [EPR6704(B)] (ab124785) -
![Anti-LSS antibody [EPR6704(B)] (ab124785)](https://www.abcam.cn/ps/products/124/ab124785/Images/ab124785-4-benefits-of-recombinant-antibodies.png)
Anti-LSS antibody [EPR6704(B)] (ab124785)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124785 被引用在 1 文献中.
- Jin Y et al. A Novel c-MET-Targeting Antibody-Drug Conjugate for Pancreatic Cancer. Front Oncol 11:634881 (2021). PubMed: 33816276