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重组RabMAb

重组Anti-LSM12抗体[EPR12282] (ab173291)

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Western blot - Anti-LSM12 antibody [EPR12282] (ab173291)
  • Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (ab173291)
  • Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (ab173291)
  • Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (ab173291)
  • Anti-LSM12 antibody [EPR12282] (ab173291)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12282] to LSM12
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-LSM12抗体[EPR12282]
    参阅全部 LSM12 一抗

  • 描述

    兔单克隆抗体[EPR12282] to LSM12

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human LSM12 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q3MHD2

  • 阳性对照

    • 293T, HepG2 and HeLa whole cell lysate (ab150035); HepG2 and 293T cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12282

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab173291于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
应用说明
Is unsuitable for IHC-P.

靶标

图片

  • Western blot - Anti-LSM12 antibody [EPR12282] (ab173291)
    Western blot - Anti-LSM12 antibody [EPR12282] (ab173291)
    All lanes : Anti-LSM12 antibody [EPR12282] (ab173291) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (ab173291)
    Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282] (ab173291)

    Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173291 at 1/100 dilution.

  • Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (ab173291)
    Flow Cytometry (Intracellular) - Anti-LSM12 antibody [EPR12282] (ab173291)

    Intracellular Flow Cytometry analysis of permeabilized 293T cells labeling LSM12 with ab173291 at 1/10 dilution (red),compared to arabbit IgG negative control (green). 

     

     

  • Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (ab173291)
    Immunoprecipitation - Anti-LSM12 antibody [EPR12282] (ab173291)

    Western blot analysis on immunoprecipitation pellet from 1) HepG2 cell lysate or 2) 1X PBS (negative control) using ab173291 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-LSM12 antibody [EPR12282] (ab173291)
    Anti-LSM12 antibody [EPR12282] (ab173291)

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