Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-LSM12抗体[EPR12282-87] (ab173292)

Submit a review Submit a question References (1)
Western blot - Anti-LSM12 antibody [EPR12282-87] (ab173292)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] (ab173292)
  • Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] (ab173292)
  • Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] (ab173292)
  • Anti-LSM12 antibody [EPR12282-87] (ab173292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12282-87] to LSM12
  • Suitable for: ICC/IF, IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-LSM12抗体[EPR12282-87]
    参阅全部 LSM12 一抗

  • 描述

    兔单克隆抗体[EPR12282-87] to LSM12

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-P, IP, WBmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Human LSM12. The exact sequence is proprietary.
    Database link: Q3MHD2

  • 阳性对照

    • 293T, HepG2, HeLa and fetal brain lysates; Human endometrial carcinoma tissue; HepG2 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR12282-87

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab173292于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
WB
1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
说明
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
WB
1/10000 - 1/50000. Predicted molecular weight: 22 kDa.
应用说明
Is unsuitable for Flow Cyt.

靶标

图片

  • Western blot - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Western blot - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    All lanes : Anti-LSM12 antibody [EPR12282-87] (ab173292) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM12 antibody [EPR12282-87] (ab173292)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LSM12 with ab173292 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Immunocytochemistry/ Immunofluorescence - Anti-LSM12 antibody [EPR12282-87] (ab173292)

    Immunofluorescent analysis of HepG2 cells labeling LSM12 with ab173292 at 1/100 dilution.

  • Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Immunoprecipitation - Anti-LSM12 antibody [EPR12282-87] (ab173292)

    Western blot analysis on immunoprecipitation pellet from 1) HeLa cell lysate or 2) 1X PBS using ab173292 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-LSM12 antibody [EPR12282-87] (ab173292)
    Anti-LSM12 antibody [EPR12282-87] (ab173292)

文献 (1)

发表研究结果有使用 ab173292？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab173292 被引用在 1 文献中.

  • Ayache J  et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell 26:2579-95 (2015). WB ; Human . PubMed: 25995375

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab173292.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com