AB236396

Anti-LSM11抗体[OTI2G7]

Name: LSM11抗体[OTI2G7] (ab236396)| Abcam中文官网
Brand: Abcam Limited
SKU: AB236396
Price: 4864 CNY
Availability: InStock

Anti-LSM11 antibody [OTI2G7]

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(1 Publication)

Mouse Monoclonal LSM11 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LSM11.

查看别名

U7 snRNA-associated Sm-like protein LSm11, LSM11

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM11 antibody [OTI2G7] (AB236396)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM11 antibody [OTI2G7] (AB236396)

Paraffin-embedded human prostate carcinoma tissue stained for LSM11 using ab236396 at 1/2000 in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM TRis buffer (pH 8.5) at 120°C for 3 minutes.

Supplier Data

Western blot - Anti-LSM11 antibody [OTI2G7] (AB236396)

All lanes:

Western blot - Anti-LSM11 antibody [OTI2G7] (ab236396) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY LSM11 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 40 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

OTI2G7

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human LSM11.

P83369

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 minutes.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

Purified from cell culture supernatant.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

LSM11 also known as U7 snRNP protein homolog is an important component in the spliceosomal snRNP complex specifically associated with U7 snRNP. LSM11 consists of a molecular mass of approximately 56 kDa. This protein expresses primarily in the nucleus where it plays a significant role in the processing of histone pre-mRNA. The mechanical action of LSM11 involves binding to RNA and facilitating the assembly of snRNP complexes which are essential in the maturation of histone transcripts.

Biological function summary

LSM11 acts within the U7 snRNP complex interacting with other snRNP proteins to ensure proper pre-mRNA cleavage and polyadenylation inhibition. This makes LSM11 essential for the correct formation of mature histone mRNA as histones require maturation without polyadenylation. The lack of poly-A tails in histone mRNA is vital for its role during DNA synthesis linking LSM11 directly to the cell cycle's intricacies. Its function is tightly regulated with multiple proteins interacting with LSM11 to modulate its activity within the histone gene expression pathway.

Pathways

LSM11 functionally integrates into the histone pre-mRNA processing pathway. This pathway critically contributes to cell cycle regulation particularly during DNA replication. LSM11 interacts with other key proteins such as FLASH and the U7 snRNA facilitating precise mRNA processing. Additionally LSM11 forms important interactions within the spliceosomal complex supporting the proper execution of splicing-related tasks necessary for cellular proliferation.

LSM11 has links to cell cycle-related abnormalities such as certain cancers where histone mRNA maturation is disrupted. Aberrant expression or functional disruption of LSM11 can lead to faulty histone mRNA processing contributing to tumorigenesis. The protein's association with other factors like FUS which also plays a role in RNA binding and processing highlights the complex interactions required to maintain normal cellular functions. Defects in LSM11 alongside disruptions in related proteins can manifest in various cancers where cell cycle regulation is impaired.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the U7 snRNP complex that is involved in the histone 3'-end pre-mRNA processing (PubMed : 11574479, PubMed : 16914750, PubMed : 33230297). Increases U7 snRNA levels but not histone 3'-end pre-mRNA processing activity, when overexpressed (PubMed : 11574479, PubMed : 16914750). Required for cell cycle progression from G1 to S phases (By similarity). Binds specifically to the Sm-binding site of U7 snRNA (PubMed : 11574479, PubMed : 16914750).

See full target information LSM11

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Digestive diseases and sciences 70:1395-1410 PubMed39961962

2025

LRRC25 Is a Potential Biomarker for Predicting Immunotherapy Response in Patients with Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhensong Yang,Bin Yu,Jinchen Hu,Lixin Jiang,Mi Jian

View all publications

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Anti-LSM11抗体[OTI2G7]

Anti-LSM11 antibody [OTI2G7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSM11 antibody [OTI2G7] (AB236396)

Western blot - Anti-LSM11 antibody [OTI2G7] (AB236396)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原