Anti-LRSAM1抗体(ab73113)
Key features and details
- Mouse polyclonal to LRSAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LRSAM1抗体
参阅全部 LRSAM1 一抗 -
描述
小鼠多克隆抗体to LRSAM1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human LRSAM1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human liver tissue lysate; LRSAM1-transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73113于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
靶标
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功能
E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. -
组织特异性
Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Charcot-Marie-Tooth disease 2P (CMT2P) [MIM:614436]: An axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Note=The disease is caused by mutations affecting the gene represented in this entry. -
序列相似性
Contains 6 LRR (leucine-rich) repeats.
Contains 1 RING-type zinc finger.
Contains 1 SAM (sterile alpha motif) domain. -
结构域
The coiled coil domains interact with the SB domain of TSG101.
The PTAP motifs mediate the binding to UEV domains. -
细胞定位
Cytoplasm. Displays a punctuate distribution and localizes to a submembranal ring. - Information by UniProt
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数据库链接
- Entrez Gene: 90678 Human
- Omim: 610933 Human
- SwissProt: Q6UWE0 Human
- Unigene: 495188 Human
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别名
- CMT2P antibody
- E3 ubiquitin protein ligase LRSAM1 antibody
- E3 ubiquitin-protein ligase LRSAM1 antibody
see all
图片
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Western blot - Anti-LRSAM1 antibody (ab73113)Anti-LRSAM1 antibody (ab73113) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
HRP-conjugated Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa -
Western blot - Anti-LRSAM1 antibody (ab73113)All lanes : Anti-LRSAM1 antibody (ab73113) at 1/500 dilution
Lane 1 : LRSAM1-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
数据表及文件
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Datasheet download
文献 (8)
ab73113 被引用在 8 文献中.
- Minaidou A et al. Deregulation of LRSAM1 expression impairs the levels of TSG101, UBE2N, VPS28, MDM2 and EGFR. PLoS One 14:e0211814 (2019). PubMed: 30726272
- Minaidou A et al. LRSAM1 Depletion Affects Neuroblastoma SH-SY5Y Cell Growth and Morphology: The LRSAM1 c.2047-1G>A Loss-of-Function Variant Fails to Rescue The Phenotype. Cell J 20:340-347 (2018). PubMed: 29845787
- Polajnar M et al. Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella. EMBO Rep 18:1572-1585 (2017). PubMed: 28784601
- Peeters K et al. Charcot-Marie-Tooth disease type 2G redefined by a novel mutation in LRSAM1. Ann Neurol 80:823-833 (2016). PubMed: 27686364
- Hu B et al. A novel missense mutation of CMT2P alters transcription machinery. Ann Neurol 80:834-845 (2016). PubMed: 27615052
- Bogdanik LP et al. Loss of the E3 ubiquitin ligase LRSAM1 sensitizes peripheral axons to degeneration in a mouse model of Charcot-Marie-Tooth disease. Dis Model Mech 6:780-92 (2013). WB . PubMed: 23519028
- Nicolaou P et al. A novel LRSAM1 mutation is associated with autosomal dominant axonal Charcot-Marie-Tooth disease. Eur J Hum Genet : (2012). Human . PubMed: 22781092
- Guernsey DL et al. Mutation in the gene encoding ubiquitin ligase LRSAM1 in patients with Charcot-Marie-Tooth disease. PLoS Genet 6: (2010). WB ; Human . PubMed: 20865121
