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AB172380

重组Anti-LRRK2 (phospho S955)抗体[MJF-R11 (75-1)] - BSA and Azide free

Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] - BSA and Azide free

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Rabbit Recombinant Monoclonal LRRK2 phospho S955 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

PARK8, LRRK2, Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin

1 Images
Western blot - Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] - BSA and Azide free (AB172380)
  • WB

Collaborator

Western blot - Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] - BSA and Azide free (AB172380)

This data was developed using ab169521, the same antibody clone in a different buffer formulation.

Lanes 1 - 2:

Anti-DDDDK tag antibody

Lanes 3 - 4:

Western blot - Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] (<a href='/products/primary-antibodies/lrrk2-phospho-s955-antibody-mjf-r11-75-1-ab169521'>ab169521</a>) at 1/2000 dilution

Lanes 5 - 6:

Western blot - Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)] (<a href='/products/primary-antibodies/lrrk2-phospho-s955-antibody-mjf-r11-75-1-ab169521'>ab169521</a>) at 1/10000 dilution

Lanes 1, 3 and 5:

HEK293T cells stably expressed with wild-type DDDDK-LRRK2

Lanes 2, 4 and 6:

HEK293T cells stably expressed with mutant DDDDK-LRRK2

Predicted band size: 286 kDa

false

This image is courtesy of Drs. Dario Alessi and Paul Davies (The University of Dundee)

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-LRRK2 (phospho S955) antibody [MJF-R11 (75-1)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

MJF-R11 (75-1)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein LRRK2 also known as leucine-rich repeat kinase 2 or dardarin is an enzyme with a molecular weight of approximately 286 kDa. It functions as a kinase meaning it adds phosphate groups to other proteins which affects their activity. LRRK2 is expressed in various tissues but it is highly abundant in the brain especially in regions such as the striatum and cortex. It has a significant role in cellular signaling processes due to its phosphorylation activity.
Biological function summary

LRRK2 interacts with cellular mechanisms by regulating cytoskeletal dynamics autophagy and vesicle trafficking. It is a part of a larger complex that includes other proteins involved in these processes. The kinase activity of LRRK2 plays an essential part in maintaining neuronal health and function. It influences the process of autophagy which is a way cells clean themselves by removing damaged components and recycling them.

Pathways

The action of LRRK2 is central to the mitogen-activated protein kinase (MAPK) and the mammalian target of rapamycin (mTOR) pathways. In these pathways LRRK2 interacts with other proteins such as mTOR and RPS6KB1. It modulates cellular processes like growth proliferation and response to stressors. Its kinase activity affects the phosphorylation state of targets within the pathways hence influencing biological outcomes like survival and apoptosis.

LRRK2 mutations have a significant connection to Parkinson's disease and Crohn's disease. In Parkinson's disease mutated LRRK2 leads to abnormal protein aggregation linking to proteins such as alpha-synuclein. For Crohn's disease LRRK2 influences the immune response and intestinal inflammation. These connections highlight LRRK2's role in the pathogenesis and contribute to understanding these complex disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The protein expressed by the gene LRRK2 is a serine/threonine-protein kinase that phosphorylates a wide range of proteins involved in neuronal plasticity, autophagy, and vesicle trafficking. It serves as a key regulator of RAB GTPases by affecting the GTP/GDP exchange and interaction partners of RABs through phosphorylation, targeting proteins like RAB3A, RAB3B, and others. LRRK2 manages the GDP/GTP exchange for RAB8A by phosphorylating 'Thr-72', inhibiting RAB8A's interaction with GDI1/GDI2, and influencing primary ciliogenesis to enhance SHH signaling in the brain. It works with RAB29 in retromer-dependent recycling of proteins between lysosomes and the Golgi. The protein is involved in shaping neuronal morphology in the CNS, synaptic vesicle trafficking, and recruiting SEC16A to assist in ER to Golgi transport. It enhances autophagy via the CaMKK/AMPK pathway and relates to nicotinic acid adenine dinucleotide phosphate receptors and lysosomal activity. LRRK2 phosphorylates PRDX3 and APP, influencing neuron apoptosis. Independently, it inhibits MAPT degradation, fostering MAPT oligomerization. Additionally, LRRK2 possesses GTPase activity that regulates its kinase activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information LRRK2 phospho S955
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