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重组RabMAb

重组Anti-LRRK2 (phospho S935)抗体[UDD2 10(12)] - BSA and Azide free (ab172382)

Submit a review Submit a question References (13)
Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
  • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
  • Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UDD2 10(12)] to LRRK2 (phospho S935) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-LRRK2 (phospho S935)抗体[UDD2 10(12)] - BSA and Azide free
    参阅全部 LRRK2 一抗

  • 描述

    兔单克隆抗体[UDD2 10(12)] to LRRK2 (phospho S935) - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    The antibody does not give a positive signal in U-87 MG, SH-SY-5Y and human fetal brain. Please contact our Scientific Support team if you have any question.

  • 经测试应用

    适用于: WBmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: GFP LRRK2, GFP LRRK2 S910A, GFP LRRK2 S935A, LRRK2 WT MEF, LRRK2 WT MEF, Lymphoblastoid, NIH/3T3 and RAW 264.7 cell lysates.

  • 常规说明

    ab172382 is the carrier-free version of ab133450.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This antibody was developed with support from The Michael J. Fox Foundation.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab172382于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).

Please check the parent abID, ab133450, for a recommended dilution.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 286 kDa (predicted molecular weight: 286 kDa).

Please check the parent abID, ab133450, for a recommended dilution.
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

  • 功能

    Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.

  • 组织特异性

    Expressed in the brain. Expressed in pyramidal neurons in all cortical laminae of the visual cortex, in neurons of the substantia nigra pars compacta and caudate putamen (at protein level). Expressed throughout the adult brain, but at a lower level than in heart and liver. Also expressed in placenta, lung, skeletal muscle, kidney and pancreas. In the brain, expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas.

  • 疾病相关

    Parkinson disease 8

  • 序列相似性

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 protein kinase domain.
    Contains 1 Roc domain.
    Contains 7 WD repeats.

  • 结构域

    The seven-bladed WD repeat region is critical for synaptic vesicle trafficking and mediates interaction with multiple vesicle-associated presynaptic proteins.
    The Roc domain mediates homodimerization and regulates kinase activity.

  • 翻译后修饰

    Autophosphorylated.

  • 细胞定位

    Membrane. Cytoplasm. Perikaryon. Mitochondrion. Golgi apparatus. Cell projection, axon. Cell projection, dendrite. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Endosome. Lysosome. Mitochondrion outer membrane. Mitochondrion inner membrane. Mitochondrion matrix. Predominantly associated with intracytoplasmic vesicular and membranous structures (By similarity). Localized in the cytoplasm and associated with cellular membrane structures. Predominantly associated with the mitochondrial outer membrane of the mitochondria. Colocalized with RAB29 along tubular structures emerging from Golgi apparatus. Localizes in intracytoplasmic punctate structures of neuronal perikarya and dendritic and axonal processes.
  • Information by UniProt

  • 数据库链接

    • 别名

      • augmented in rheumatoid arthritis 17 antibody
      • AURA17 antibody
      • Dardarin antibody
      • Leucine rich repeat kinase 2 antibody
      • leucine rich repeat serine threonine protein kinase 2 antibody
      • Leucine-rich repeat serine/threonine-protein kinase 2 antibody
      • LRRK 2 antibody
      • LRRK2 antibody
      • LRRK2_HUMAN antibody
      • PARK 8 antibody
      • PARK8 antibody
      • RIPK7 antibody
      • ROCO 2 antibody
      • ROCO2 antibody
      see all

    图片

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382) at 1/10000 dilution

      Lane 1 : Wide type-LRRK2 overexpressed lysates
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
      Lane 3 : RAW 264.7(Mouse Abelson murine leukemia virus-induced tumor macrophage)whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 286 kDa



      Exposure:
      Lane 1 and 2: 5 seconds.
      Lane 3: 180 seconds.

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/5000 dilution

      Lane 1 : WT-LRRK2 cell lysate - untreated
      Lane 2 : WT-LRRK2 cell lysate - treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 286 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

    • Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      Western blot - Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      All lanes : Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] (ab133450) at 1/1000 dilution (unpurified)

      Lane 1 : GFP LRRK2 lysate at 5 µg
      Lane 2 : GFP LRRK2 S910A lysate at 5 µg
      Lane 3 : GFP LRRK2 S935A lysate at 5 µg
      Lane 4 : LRRK2 WT MEF lysate at 20 µg
      Lane 5 : LRRK2 WT MEF lysate from LRRK2 IN1 treated cells at 20 µg
      Lane 6 : LRRK2 KO MEF lysate at 20 µg
      Lane 7 : LRRK2 KO MEF lysate from LRRK2 IN1 treated cells at 20 µg
      Lane 8 : Lymphoblastoid lysate at 30 µg
      Lane 9 : Lymphoblastoid lysate from LRRK2 IN1 treated cells at 30 µg

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 286 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133450).

    • Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)
      Anti-LRRK2 (phospho S935) antibody [UDD2 10(12)] - BSA and Azide free (ab172382)

    文献 (13)

    发表研究结果有使用 ab172382？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab172382 被引用在 13 文献中.

    • Obergasteiger J  et al. The small GTPase Rit2 modulates LRRK2 kinase activity, is required for lysosomal function and protects against alpha-synuclein neuropathology. NPJ Parkinsons Dis 9:44 (2023). PubMed: 36973269
    • Iannotta L  et al. Divergent Effects of G2019S and R1441C LRRK2 Mutations on LRRK2 and Rab10 Phosphorylations in Mouse Tissues. Cells 9:N/A (2020). PubMed: 33105882
    • Obergasteiger J  et al. Kinase inhibition of G2019S-LRRK2 enhances autolysosome formation and function to reduce endogenous alpha-synuclein intracellular inclusions. Cell Death Discov 6:45 (2020). PubMed: 32550012
    • Bae EJ  et al. LRRK2 kinase regulates a-synuclein propagation via RAB35 phosphorylation. Nat Commun 9:3465 (2018). PubMed: 30150626
    • Andersen MA  et al. PFE-360-induced LRRK2 inhibition induces reversible, non-adverse renal changes in rats. Toxicology 395:15-22 (2018). PubMed: 29307545
    View all Publications for this product

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