Anti-LRRFIP1抗体(ab176587)
Key features and details
- Rabbit polyclonal to LRRFIP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LRRFIP1抗体
参阅全部 LRRFIP1 一抗 -
描述
兔多克隆抗体to LRRFIP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human LRRFIP1 aa 758-808 (C terminal). The exact sequence is proprietary. (NP_001131024.1).
Sequence:QTVRKALDSNSLENDDLSAPGREPGHFNPESREDTRGGNEKGKSKEDCTM S
Database link: Q32MZ4 -
阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176587 was affinity purified using an epitope specific to LRRFIP1 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176587于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 89 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 89 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the LRRFIP family. -
发展阶段
Widely expressed in fetal tissues. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9208 Human
- Omim: 603256 Human
- SwissProt: Q32MZ4 Human
- Unigene: 471779 Human
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别名
- FLAP 1 antibody
- FLAP1 antibody
- FLIIAP 1 antibody
see all
图片
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Immunoprecipitation - Anti-LRRFIP1 antibody (ab176587)
Detection of LRRFIP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176587 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab176587 was used at 0.4 µg/ml. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 1 second.
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Western blot - Anti-LRRFIP1 antibody (ab176587)All lanes : Anti-LRRFIP1 antibody (ab176587) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 89 kDa
Exposure time: 30 seconds
数据表及文件
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Datasheet download
文献 (0)
ab176587 尚未被引用在任何文献中。
