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AB224244

Anti-LPCAT-2抗体

Anti-LPCAT-2 antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal LPCAT-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LPCAT2 aa 400 to C-terminus.

查看别名

AGPAT11, AYTL1, LPCAT2, Lysophosphatidylcholine acyltransferase 2, LPC acyltransferase 2, LPCAT-2, LysoPC acyltransferase 2, 1-acylglycerol-3-phosphate O-acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acyltransferase-like 1, Lysophosphatidic acid acyltransferase alpha, 1-AGP acyltransferase 11, 1-AGPAT 11, Acetyl-CoA:lyso-PAF acetyltransferase, Lyso-PAF acetyltransferase, LysoPAFAT, LPAAT-alpha

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-LPCAT-2 antibody (AB224244)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LPCAT-2 antibody (AB224244)

A431 (human epidermoid carcinoma cell line) cells stained for LPCAT-2 (green) using ab224244 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT-2 antibody (AB224244)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT-2 antibody (AB224244)

Paraffin embedded human thyroid gland tissue stained for LPCAT-2 with ab224244 (1/50 dilution) in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT-2 antibody (AB224244)
  • IHC-P

AbReview79406****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT-2 antibody (AB224244)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed mouse liver tissue. Stained with ab224244 at 1/100 dilution. Secondary antibody used OmniMap anti-Rabbit HRP at 1/200 dilution. Blocking was done with 10% BSA for 1 hour 30 minutes at 37°C. The sample was incubated with the primary antibody for 1 hour at 37°C.

This image is courtesy of an anonymous Abreview

Western blot - Anti-LPCAT-2 antibody (AB224244)
  • WB

Supplier Data

Western blot - Anti-LPCAT-2 antibody (AB224244)

All lanes:

Western blot - Anti-LPCAT-2 antibody (ab224244) at 1/100 dilution

All lanes:

Human thyroid gland tissue lysate

Predicted band size: 60 kDa

false

Western blot - Anti-LPCAT-2 antibody (AB224244)
  • WB

AbReview79407****

Western blot - Anti-LPCAT-2 antibody (AB224244)

Blocking was done with 3% Milk for 1 hour 15 mins at 37°C

All lanes:

Western blot - Anti-LPCAT-2 antibody (ab224244) at 1/400 dilution

All lanes:

Mouse liver tissue lysate at 10 µg

Secondary

All lanes:

Invitrogen anti-Rabbit HRP at 1/1000 dilution

true

Exposure time: 27hr

This image is courtesy of an anonymous Abreview

Western blot - Anti-LPCAT-2 antibody (AB224244)
  • WB

Supplier Data

Western blot - Anti-LPCAT-2 antibody (AB224244)

All lanes:

Western blot - Anti-LPCAT-2 antibody (ab224244) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat cell line) cell lysate

Predicted band size: 60 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human LPCAT2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7L5N7

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Exhibits both acyltransferase and acetyltransferase activities (PubMed : 17182612, PubMed : 20363836, PubMed : 21498505). Catalyzes the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed : 21498505). Catalyzes the conversion 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed : 20363836). Involved in platelet-activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF) (PubMed : 17182612). Also converts lyso-PAF to 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine (PC), a major component of cell membranes and a PAF precursor (By similarity). Under resting conditions, acyltransferase activity is preferred (By similarity). Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases (By similarity). Involved in the regulation of lipid droplet number and size (PubMed : 25491198).
See full target information LPCAT2

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:5772 PubMed34599178

2021

Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Fabien Thery,Lia Martina,Caroline Asselman,Yifeng Zhang,Madeleine Vessely,Heidi Repo,Koen Sedeyn,George D Moschonas,Clara Bredow,Qi Wen Teo,Jingshu Zhang,Kevin Leandro,Denzel Eggermont,Delphine De Sutter,Katie Boucher,Tino Hochepied,Nele Festjens,Nico Callewaert,Xavier Saelens,Bart Dermaut,Klaus-Peter Knobeloch,Antje Beling,Sumana Sanyal,Lilliana Radoshevich,Sven Eyckerman,Francis Impens
View all publications

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