重组Anti-LOXL2抗体[EPR12733] - C-terminal

• WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

Lanes 1-4 : Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LOXL2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LOXL2 knockout HeLa cell line (<a href='/products/cell-lines/human-loxl2-knockout-hela-cell-line-ab261804'>ab261804</a>)

Lane 3:

HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

Exposure time :

Lane 1-3 : 3min
Lane 4 : 1min

Blocking/Diluting buffer and concentration 5% NFDM /TBST

MCF-7 lack LOXL2 expression (PMID : 19330836, PMID : 12154058 and PMID : 27655685)

Lanes 1, 2 and 4:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/1000 dilution

Lane 3:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/200 dilution

Lane 1:

MCF-7 (Human breast carcinoma) whole cell lysate at 10 µg

Lane 2:

MDA-MB-435 (Human ductal carcinoma) whole cell lysate at 10 µg

Lane 3:

A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Lane 4:

U87-MG (Human glioblastoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

Lanes 1-4 : Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264807 (knockout cell lysate ab257166) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LOXL2 knockout HeLa cell lysate at 20 µg

Lane 3:

HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

Lanes 1-4 : Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261804 (knockout cell lysate ab257168) was used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (ab179810) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LOXL2 knockout HeLa cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 105 kDa

false