重组Anti-LOX 1抗体[EPR27871-67] (ab317689)
![Western blot - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-542219-anti-lox-1-antibody-epr27871-67-western-blot-mouse-placenta-tissue-lysate-20-ug.jpg "Western blot - Anti-LOX 1 antibody [EPR27871-67] (ab317689)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27871-67] to LOX 1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
概述
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产品名称
Anti-LOX 1抗体[EPR27871-67]
参阅全部 LOX 1 一抗 -
描述
兔单克隆抗体[EPR27871-67] to LOX 1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Rat
不与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse placenta, Untreated Mouse placenta, Mouse placenta treated with Peptide:N-glycosidase F and Rat placenta tissue lysates. IHC-P: Mouse placenta, Mouse spleen and Rat placenta tissues. IP: Mouse placenta tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27871-67 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317689于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Predicted molecular weight: 31 kDa.
|
|
| IP |
1/30.
|
| 说明 |
|---|
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 31 kDa. |
|
IP
1/30. |
靶标
-
功能
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
组织特异性
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
疾病相关
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
序列相似性
Contains 1 C-type lectin domain. -
结构域
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
翻译后修饰
The intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
细胞定位
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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数据库链接
- Entrez Gene: 108078 Mouse
- Entrez Gene: 140914 Rat
- SwissProt: Q9EQ09 Mouse
- SwissProt: O70156 Rat
- Unigene: 293626 Mouse
- Unigene: 87449 Rat
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别名
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
see all
图片
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Western blot - Anti-LOX 1 antibody [EPR27871-67] (ab317689)All lanes : Anti-LOX 1 antibody [EPR27871-67] (ab317689) at 1/1000 dilution
Lane 1 : Mouse placenta tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Untreated Mouse placenta tissue lysate
Lane 6 : Mouse placenta tissue lysate treated with Peptide:N-glycosidase F (PNGase F)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 30-37 kDa why is the actual band size different from the predicted?
Additional bands at: 50-75 kDa (possible glycosylated form)
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: liver, skeletal muscle, brain (PMID: 9828121, PMID: 9052782).
LOX1 is a glycoprotein of approximately 50-75 kDa and detected as a 30-37 kDa band after treated with Peptide:N-glycosidase F (PNGase F).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9052782, PMID: 12354387).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-542220-anti-lox-1-antibody-epr27871-67-western-blot-rat-placenta-tissue-lysate-20-ug.jpg)
Western blot - Anti-LOX 1 antibody [EPR27871-67] (ab317689)All lanes : Anti-LOX 1 antibody [EPR27871-67] (ab317689) at 1/1000 dilution
Lane 1 : Rat placenta tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 30-37 kDa why is the actual band size different from the predicted?
Additional bands at: 50-75 kDa (possible glycosylated form)
Exposure time: 37 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: liver (PMID: 9828121, PMID: 9052782).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9052782, PMID: 12354387).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-1-anti-lox-1-antibody-epr27871-67-immunohistochemistry-mouse-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)Immunohistochemical analysis of paraffin-embedded Mouse placenta tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in trophoblast of mouse placenta (PMID: 18474940).
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-2-anti-lox-1-antibody-epr27871-67-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in immune cell of mouse spleen.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-3-anti-lox-1-antibody-epr27871-67-immunohistochemistry-rat-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)Immunohistochemical analysis of paraffin-embedded Rat placenta tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in trophoblast of rat placenta.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-4-anti-lox-1-antibody-epr27871-67-immunohistochemistry-mouse-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining in mouse skeletal muscle.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-5-anti-lox-1-antibody-epr27871-67-immunohistochemistry-rat-skeletal-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX 1 antibody [EPR27871-67] (ab317689)Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling LOX 1 with ab317689 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining in rat skeletal muscle.
The section was incubated with ab317689 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
-
![Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-6-anti-lox-1-antibody-epr27871-67-immunoprecipitation-mouse-placenta-tissue-lysate.jpg)
Immunoprecipitation - Anti-LOX 1 antibody [EPR27871-67] (ab317689)LOX 1 was immunoprecipitated from 0.35 mg Mouse placenta tissue lysate with ab317689 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317689 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse placenta tissue lysate
Lane 2: ab317689 IP in Mouse placenta tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317689 in mouse placenta tissue lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9052782, PMID: 12354387).
-
![Anti-LOX 1 antibody [EPR27871-67] (ab317689)](https://www.abcam.cn/ps/products/317/ab317689/Images/ab317689-7-benefitsofrecombinantantibodies.jpg)
Anti-LOX 1 antibody [EPR27871-67] (ab317689)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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