重组Anti-Loricrin抗体[EPR7148(2)(B)] (ab176322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7148(2)(B)] to Loricrin
- Suitable for: IHC-P, WB, mIHC
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Loricrin抗体[EPR7148(2)(B)]
参阅全部 Loricrin 一抗 -
描述
兔单克隆抗体[EPR7148(2)(B)] to Loricrin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, mIHCmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Loricrin aa 1-100. The exact sequence is proprietary.
Database link: P23490 -
阳性对照
- Human skin lysate and Human skin tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7148(2)(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
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mIHC |
1/3000.
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说明 |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa. |
mIHC
1/3000. |
靶标
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功能
Major keratinocyte cell envelope protein. -
疾病相关
Defects in LOR are a cause of progressive symmetric erythrokeratodermia (PSEK) [MIM:133200]. Erythrokeratodermas are a group of disorders characterized by widespread erythematous plaques, either stationary or migratory, associated with features that include palmoplantar keratoderma. PSEK is characterized by erythematous and hyperkeratotic plaques.
Defects in LOR are the cause of Vohwinkel syndrome with ichthyosis (VSI) [MIM:604117]; also known as loricrin keratoderma (LK) or mutilating keratoderma with ichthyosis. VSI is an ichthyotic variant of Vohwinkel syndrome (VS) characterized by progressive symmetric erythrokeratoderma or congenital ichthyosiform erythroderma born as a collodion baby. Common clinical features include hyperkeratosis of the palms and soles with digital constriction. -
翻译后修饰
Substrate of transglutaminases. Some glutamines and lysines are cross-linked to other loricrin molecules and to SPRRs proteins.
Contains inter- or intramolecular disulfide-bonds. -
细胞定位
Cytoplasm. Nucleus > nucleoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4014 Human
- Omim: 152445 Human
- SwissProt: P23490 Human
- Unigene: 251680 Human
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别名
- LOR antibody
- LOR protein antibody
- LORI_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue labelling Aquaporin 3 with ab307969 at 1/5000 dilution (0.094 µg/mL) (B), Loricrin with ab176322 at 1/3000 dilution (0.031 μg/ml) (C) and Filaggrin with ab221155 at 1/2000 dilution (0.523 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Aquaporin 3 (magenta; Opal™690), anti-Loricrin (green; Opal™520) and anti-Filaggrin (red; Opal™570) on human skin.
Panel B: anti-Aquaporin 3 stained on keratinocytes from the stratum basale to the stratum granulosum of the epidermis.
Panel C: anti-Loricrin stained on stratum corneum.
Panel D: anti-Filaggrin stained on the granular to stratum corneum transition.The section was incubated in three rounds of staining: in the order of ab307969, ab176322, and ab221155 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Anti-Loricrin antibody [EPR7148(2)(B)] (ab176322) at 1/10000 dilution + Human skin lysate at 10 µg
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Loricrin using ab176322 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab176322 被引用在 8 文献中.
- Cadau S et al. An Inflamed and Infected Reconstructed Human Epidermis to Study Atopic Dermatitis and Skin Care Ingredients. Int J Mol Sci 23:N/A (2022). PubMed: 36361668
- Rizaldy D et al. Increase in primary cilia in the epidermis of patients with atopic dermatitis and psoriasis. Exp Dermatol 30:792-803 (2021). PubMed: 33455013
- Rahim AB et al. The Polyamine Regulator AMD1 Upregulates Spermine Levels to Drive Epidermal Differentiation. J Invest Dermatol 141:2178-2188.e6 (2021). PubMed: 33984347
- Xu D & Wang J Downregulation of Cathepsin B Reduces Proliferation and Inflammatory Response and Facilitates Differentiation in Human HaCaT Keratinocytes, Ameliorating IL-17A and SAA-Induced Psoriasis-Like Lesion. Inflammation N/A:N/A (2021). PubMed: 34037897
- Griffoni C et al. In vitro skin culture media influence the viability and inflammatory response of primary macrophages. Sci Rep 11:7070 (2021). PubMed: 33782484
- Becker M et al. WIF1 Suppresses the Generation of Suprabasal Cells in Acanthotic Skin and Growth of Basal Cell Carcinomas upon Forced Overexpression. J Invest Dermatol 140:1556-1565.e11 (2020). PubMed: 31987884
- Tan CL et al. Nicotinamide Metabolism Modulates the Proliferation/Differentiation Balance and Senescence of Human Primary Keratinocytes. J Invest Dermatol 139:1638-1647.e3 (2019). PubMed: 30776433
- Tang Y et al. RNF31 Regulates Skin Homeostasis by Protecting Epidermal Keratinocytes from Cell Death. J Immunol 200:4117-4124 (2018). PubMed: 29728512