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重组RabMAb

重组Anti-LONP1/Lon抗体[EPR28510-62] - BSA and Azide free (ab317562)

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Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
  • Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28510-62] to LONP1/Lon - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-LONP1/Lon抗体[EPR28510-62] - BSA and Azide free
    参阅全部 LONP1/Lon 一抗

  • 描述

    兔单克隆抗体[EPR28510-62] to LONP1/Lon - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IP, WBmore details
    不适用于: Flow Cyt (Intra) or ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HeLa transfected with siRNA specifically targeting LONP1/Lon, 293T, PC-12, Neuro-2a, U-87 MG, SH-SY5Y, NIH/3T3, C6, Rat liver, C2C12 (mouse myoblast), Mouse liver, Mouse thymus, Human liver and human placenta lysates. IHC-P: Human lung, Human colon, Human colon carcinoma, Mouse lung, Mouse colon, Mouse lung cancer, Rat lung and Rat colon tissues. IP: HeLa cell.

  • 常规说明

    ab317562 is the carrier-free version of ab317561.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317562于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 106 kDa.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 106 kDa.
应用说明
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

靶标

  • 功能

    ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters. Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein.

  • 组织特异性

    Duodenum, heart, lung and liver, but not thymus.

  • 疾病相关

    CODAS syndrome

  • 序列相似性

    Belongs to the peptidase S16 family.
    Contains 1 Lon domain.

  • 细胞定位

    Mitochondrion matrix.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • hLON antibody
    • hLON ATP dependent protease antibody
    • LON antibody
    • lon peptidase 1, mitochondrial antibody
    • LON protease antibody
    • Lon protease homolog antibody
    • Lon protease like protein antibody
    • Lon protease-like protein antibody
    • LONHs antibody
    • LONM_HUMAN antibody
    • LONP antibody
    • Lonp1 antibody
    • MGC1498 antibody
    • mitochondrial antibody
    • Mitochondrial ATP dependent protease Lon antibody
    • Mitochondrial ATP-dependent protease Lon antibody
    • Mitochondrial lon peptidase 1 antibody
    • PIM1 antibody
    • Protease serine 15 antibody
    • PRSS15 antibody
    • Serine protease 15 antibody
    see all

图片

  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    All lanes : Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/1000 dilution

    Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
    Lane 2 : HeLa transfected with siRNA specifically targeting LONP1/Lon whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 10 seconds


    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    All lanes : Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/1000 dilution

    Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 5 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa


    Exposure time: 15 seconds


    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA.

  • Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Western blot - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    All lanes : Anti-LONP1/Lon antibody [EPR28510-62] (ab317561) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat thymus tissue lysate
    Lane 5 : C2C12 (mouse myoblast)
    Lane 6 : Mouse liver tissue lysate
    Lane 7 : Mouse thymus tissue lysate
    Lane 8 : Human liver tissue lysate
    Lane 9 : Human placenta tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 8-9 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 106 kDa



    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: thymus (PMID: 8119403).

    Lanes 1-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 and lane 8-9 is applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

    Bands observed below the major band at 106kDa represent the isoforms of LONP1/Lon and can be knocked down using siRNA.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-8 : 10 seconds; Lane 9: 3 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon carcinoma. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond� Polymer Refine Detection).

    Positive staining on mouse lung. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung cancer. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat lung. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling LONP1/Lon with ab317561 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon. The section was incubated with ab317561 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

    This data was developed using ab317561, the same antibody clone in a different buffer formulation.

    LONP1/Lon was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: ab317561 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317561 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    Bands observed below the major band at 106kDa in lane2 represent the isoforms of LONP1/Lon.

  • Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)
    Anti-LONP1/Lon antibody [EPR28510-62] - BSA and Azide free (ab317562)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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