Anti-LOK抗体(ab70484)
Key features and details
- Rabbit polyclonal to LOK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LOK抗体
参阅全部 LOK 一抗 -
描述
兔多克隆抗体to LOK -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between C terminal amino acids 900-968 of Human LOK
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阳性对照
- Whole cell lysate from HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70484于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/15000. Predicted molecular weight: 112 kDa.
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| IP |
Use at 1-4 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/15000. Predicted molecular weight: 112 kDa. |
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IP
Use at 1-4 µg/mg of lysate. |
靶标
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功能
Can act on substrates such as myelin basic protein and histone 2A on serine and threonine residues. -
组织特异性
Expressed predominantly in lymphoid organs. -
序列相似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. - Information by UniProt
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数据库链接
- Entrez Gene: 6793 Human
- Entrez Gene: 20868 Mouse
- Omim: 603919 Human
- SwissProt: O94804 Human
- SwissProt: O55098 Mouse
- Unigene: 519756 Human
- Unigene: 8235 Mouse
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别名
- LOK antibody
- Lymphocyte oriented kinase antibody
- Lymphocyte-oriented kinase antibody
see all
图片
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Western blot - Anti-LOK antibody (ab70484)All lanes : Anti-LOK antibody (ab70484) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 112 kDa
Chemiluminescence with exposure times of 1 to 2 minutes. -
Immunoprecipitation - Anti-LOK antibody (ab70484)Detection of Human LOK by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70484 at 1 µg/mg lysate for IP.
Lane 1: input of Human LOK at 50µg
Lane 2: Other anti-human LOK antibody
Lane 3: Other anti-human LOK antibody plus blocking peptide
Lane 4: ab70484
Lane 5: ab70484 plus blocking peptide
Subsequent Western blot LOK detection was performed with 0.1 µg/mg ab70484.
Chemiluminescence with exposure times of 1 to 2 minutes.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab70484 被引用在 2 文献中.
- Zhang L et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
- Zhang L et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313