Anti-LNP抗体(ab121416)
Key features and details
- Rabbit polyclonal to LNP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-LNP抗体 -
描述
兔多克隆抗体to LNP -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 254-375 of Human LNP (Q9C0E8).
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阳性对照
- IHC-P: Human tonsil, cerebellum, testis and cervix tissues. WB: U-138 MG cell lysate. ICC: U-251 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121416于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in limb and central nervous system development. -
序列相似性
Belongs to the lunapark family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 80856 Human
- SwissProt: Q9C0E8 Human
- Unigene: 209561 Human
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别名
- lnp antibody
- LNP_HUMAN antibody
- Protein lunapark antibody
图片
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Western blot - Anti-LNP antibody (ab121416)All lanes : Anti-LNP antibody (ab121416) at 0.4 µg/ml
Lane 1 : U-138 MG (human glioblastoma cell line) transfected with control siRNA, whole cell lysate
Lane 2 : U-138 MG transfected with target specific siRNA probe #1, whole cell lysate
Lane 3 : U-138 MG transfected with target specific siRNA probe #2, whole cell lysate
Predicted band size: 48 kDaLoading Control: Anti-GAPDH
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (ab121416)Immunohistochemical analysis of human tonsil tissue labeling LNP with ab121416 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-LNP antibody (ab121416)Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121416 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (ab121416)Immunohistochemical analysis of human cervix tissue labeling LNP with ab121416 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (ab121416)Immunohistochemical analysis of human testis tissue labeling LNP with ab121416 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LNP antibody (ab121416)Immunohistochemical analysis of human cerebellum tissue labeling LNP with ab121416 at 1/500 dilution.
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Western blot - Anti-LNP antibody (ab121416)Anti-LNP antibody (ab121416) at 0.04 µg/ml + U-138 MG cell lysate
Performed under reducing conditions.
Predicted band size: 48 kDaWestern blot analysis of human U-138 MG cell lysate labeling LNP with ab121416 at 0.04 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab121416 被引用在 2 文献中.
- Akopian D et al. Co-adaptor driven assembly of a CUL3 E3 ligase complex. Mol Cell 82:585-597.e11 (2022). PubMed: 35120648
- McGourty CA et al. Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor. Cell 167:525-538.e14 (2016). PubMed: 27716508