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使用敲除细胞株进行验证重组RabMAb

重组Anti-LMAN1抗体[EPR6980] (ab126720)

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Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
  • Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6980] (ab126720)
  • OI-RD Scanning - Anti-LMAN1 antibody [EPR6980] (ab126720)
  • Anti-LMAN1 antibody [EPR6980] (ab126720)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6980] to LMAN1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-LMAN1抗体[EPR6980]
    参阅全部 LMAN1 一抗

  • 描述

    兔单克隆抗体[EPR6980] to LMAN1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HEK-293T, HeLa, NCI-H460 and JAR cell lysates. IHC-P: Papillary adenocarcinoma tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126720于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.

  • 组织特异性

    Ubiquitous.

  • 疾病相关

    Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1) [MIM:227300]; also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal.

  • 序列相似性

    Contains 1 L-type lectin-like domain.

  • 翻译后修饰

    The N-terminal may be partly blocked.

  • 细胞定位

    Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Endoplasmic reticulum golgi intermediate compartment protein 53 antibody
    • ER-Golgi intermediate compartment 53 kDa protein antibody
    • ERGIC-53 antibody
    • ERGIC53 antibody
    • ERGIC53 like protein antibody
    • F5F8D antibody
    • FMFD1 antibody
    • Gp58 antibody
    • Intracellular mannose specific lectin antibody
    • Intracellular mannose-specific lectin MR60 antibody
    • Lectin mannose binding 1 antibody
    • Lectin mannose-binding 1 antibody
    • Lman1 antibody
    • LMAN1 like protein antibody
    • LMAN1_HUMAN antibody
    • MCFD1 antibody
    • MR60 antibody
    • Protein ERGIC-53 antibody
    see all

图片

  • Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    All lanes : Anti-LMAN1 antibody [EPR6980] (ab126720) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : LMAN1 knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab126720 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab126720 Anti-LMAN1 antibody [EPR6980] was shown to specifically react with Protein ERGIC-53 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266248 (knockout cell lysate ab257505) was used. Wild-type and Protein ERGIC-53 knockout samples were subjected to SDS-PAGE. ab126720 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°CC at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Western blot - Anti-LMAN1 antibody [EPR6980] (ab126720)
    All lanes : Anti-LMAN1 antibody [EPR6980] (ab126720) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : NCI-H460 cell lysates
    Lane 3 : JAR cell lysates

    Predicted band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMAN1 antibody [EPR6980] (ab126720)

    ab126720, at 1/50 dilution, stains LMAN1 in paraffin-embedded papillary adenocarcinoma tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-LMAN1 antibody [EPR6980] (ab126720)
    OI-RD Scanning - Anti-LMAN1 antibody [EPR6980] (ab126720)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-LMAN1 antibody [EPR6980] (ab126720)
    Anti-LMAN1 antibody [EPR6980] (ab126720)

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